EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2, Bioti - 50 µg

EpiDyne Remodeling Assay Substrate DNA ST601-GATC1,2, Biotinylated is a 217 base-pair double-stranded DNA fragment containing a 5’ biotin-TEG group and two GATC restriction enzyme sites. This sequence includes the 601 nucleosome positioning sequence [1], which has high affinity for histone octamers and is useful for nucleosome assembly. The DNA also includes a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling and a 5’ biotin-TEG group. When assembled onto a nucleosome, the GATC sites are shielded within the 601 sequence but can be made accessible for restriction enzyme cleavage after nucleosome remodeling. See the EpiDyne Nucleosome Remodeling Assay Tech Note (Restriction Enzyme Assay) for more information: epicypher.com/resources/technical-notes/