E.Z.N.A.® Plasmid DNA Mini Kit II - 200 - Preps

This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.
Product Details
Downstream Application: Cloning; sanger sequencing etc.
Starting material: 5-15 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type: High-copy, low-copy, cosmid DNA
Processing mode: Manual, centrifugation or vacuum
Throughput: 1 – 24
DNA binding technology: Silica Mini Spin Column II (2x capacity of mini spin column)
Lysate clearance method: Centrifugation
Processing time: <30 minutes
Yield: 40-70 µg for high copy-number; 1-15 µg for low copy-number