E.Z.N.A.® HP Plant & Fungal DNA Kit - 5 - Preps

The E.Z.N.A.® High Performance (HP) Plant & Fungal DNA Kit is designed for efficientrecovery of genomic DNA from fresh, frozen, and dried plant or fungal tissue samplesrich in polysaccharides or having lower DNA content. Up to 100 mg wet tissue (or 50 mgdry tissue) can be processed in less than 1 hour. The system combines the reversiblenucleic acid-binding properties of the HiBind® matrix with the speed and versatility ofspin column technology to eliminate polysaccharides, phenolic compounds, and enzymeinhibitors from plant or fungal tissue lysates. Purified DNA is suitable for PCR, restrictiondigestion, and hybridization techniques. If using the E.Z.N.A.® HP Plant & Fungal DNA Kit for the first time, please read this bookletto become familiar with the procedures. This procedure relies on the well establishedproperties of the cationic detergent, cetyltrimethyl ammonium bromide (CTAB), inconjunction with the selective DNA binding of Omega Bio-tek’s HiBind® matrix, to purifyhigh-quality DNA. Samples are homogenized and lysed in a high salt buffer containingCTAB and extracted with chloroform to remove polysaccharides and other componentsthat interfere with many routine DNA isolations and downstream applications. Bindingconditions are adjusted and DNA is further purified using HiBind ® DNA Mini Columns.In this way, salts, proteins, and other contaminants are removed to yield high-qualitygenomic DNA suitable for downstream applications such as endonuclease digestion,thermal cycle amplification, and hybridization.
Product Details
Starting Amount: 100 mg of wet sample or 30 mg dry sample
Starting Material: Fresh and dried plant tissue samples rich in lipids, polyphenols, polysaccharides, or those with lower DNA content.
Elution Volume: 50-100 μL
Technology: HiBind® DNA Mini Column
Processing Mode: Manual
Throughput: 1-24