E.Z.N.A.® FastFilter Plasmid DNA Midi Kit - 2 - Preps

The E.Z.N.A.® FastFilter Plasmid DNA Midi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high quality plasmid DNA. The yields vary according to plasmid copy number,E. colistrain, and growth conditions. 15-50 mL of bacterial cultures in LB medium typically produces up to 200 µg of high copy number plasmid DNA. Up to 100 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR,in vitrotranscription, transformation, and other applications.
Product Details
Downstream Application: Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material: 15-50 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type: High-copy, low-copy, cosmid DNA
Processing mode: Manual (centrifugation or vacuum)
Throughput: 1 – 24
DNA binding technology: Silica Midi Spin Column
Lysate clearance method: Filtration using Lysate clearance syringe
Processing time: <40 minutes
Yield: 100-250 µg for high copy-number; 10-50 µg for low copy-number
Special note: Lysate clearance syringe for rapid sample processing