E.Z.N.A.® Endo-free Plasmid DNA Mini Kit II - 5 - Preps

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.
Product Details
Downstream Application: Sensitive cell line transfection; gene therapy etc.
Starting material: 5- 15 mL mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type: High-copy, low-copy, cosmid DNA
Processing mode: Manual, centrifugation
Throughput: 1 – 24
DNA binding technology: Silica Mini Spin Column II (2x capacity of mini spin column)
Lysate clearance method: Centrifugation
Processing time: <30 minutes
Yield: 40-70 µg for high copy-number; 1-15 µg for low copy-number
Special note: Includes endotoxin removal step