Empowering Researchers with Reproducible, High-Quality 3D Cell Culture Models
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Through Tebubio, you can establish a xeno-free, highly reproducible platform for advanced 3D cell culture using the VitroPrime™ Line Plates from our partner TheWell Bisocience. Designed to prioritise your workflow efficiency, data reliability, and experimental consistency, these plates enable seamless integration of culture, staining, and imaging, all within a single vessel. |
VitroPrime™ 3D Culture and Imaging Plate
Premium 3D cell culture plates with a unique media-exchange channel and “sample-locking” technology, combined with an ultra-clear cover-glass bottom, support zero-disruption 3D cell culture workflows. Perform culture, media changes, washing, staining, and imaging without transferring samples, reducing sample loss, preserving delicate 3D structures, and simplifying handling. Fully compatible with automated systems, these plates are designed for modern, high-throughput laboratories.
Key Benefits for Your Research:
- Streamlined in-plate workflow eliminates sample transfer.
- Lateral media-exchange channel ensures passive, disturbance-free diffusion.
- Anti-floating and anti-rotation design maintains positional stability of 3D constructs.
- Ultra-clear cover-glass bottom optimised for high-resolution and confocal imaging.
- Compatible with automated liquid-handling and high-throughput platforms.
- Available in 6-, 12-, and 96-well formats for flexible experimental design.
How It Works
1. Mechanical Anchoring for Precision Tracking
Unlike standard plates, VitroPrime™ securely anchors gels within a built-in retention compartment. This prevents floating or rotation, keeping organoids stable for reliable live-cell imaging and long-term studies.
The right illustration demonstrates how VitroPrime™ maintains gel integrity compared with standard plates, ensuring consistent 3D structure tracking over time.
2. Zero-Disruption Automation and Medium Exchange
Each well includes a lateral medium-exchange channel that gently diffuses nutrients and reagents without disturbing the hydrogel. This protects delicate 3D structures during automated high-throughput workflows, including media changes, fixation, and staining.
The left illustration highlights the difference in spheroid integrity during automated handling between VitroPrime™ and conventional plates.
3. Optimised Optical Performance
The ultra-clear #1.5 (0.17 mm) cover-glass bottom maximises light transmission and minimises auto-fluorescence for superior imaging. Ideal for confocal microscopy, high-content screening, and advanced imaging with immersion objectives.
Comparison of VitroPrime™ 3D Culture and Imaging Plate vs. Conventional Culture Plates
VitroPrime™ Ultra-Low Attachment, U-Bottom, 96-Well Plate
The VitroPrime™ features a surface treatment that prevents cell adhesion, promoting uniform spheroid formation, essential for organoid and tumoroid studies. Coupled with the VitroGel® hydrogel system, researchers can reliably model spheroid invasion and advanced tumoroid assays.
Benefits for Your Experiments:
- Uniform coating ensures consistent spheroid formation across wells.
- Ideal for drug screening, high-throughput applications, and advanced 3D models.
- Designed for xeno-free systems, aligning with translational research standards.
Figure 1: Comparison of Spheroid Formation. Glioblastoma cells cultured in VitroPrime™ Ultra-Low Attachment, U-bottom, 96-well Plate formed single, uniform spheroids with no residual cells on the edges (first column). In contrast, commercially available plates produced irregular, multi-cluster spheroids (second and third columns), potentially compromising spheroid invasion assays.
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Supporting Your Full Workflow
The VitroPrime™ line also complements other 3D and 2D culture systems.
For 2D experiments, an appropriate coating substrate (e.g., vitronectin or fibronectin) is recommended to ensure optimal cell adhesion and performance.
Selecting the right matrix is critical for reproducibility and experimental success. Speak with our experts early in your project to avoid delays and costly setbacks.
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Reference
Article content created by Tebubio using courtesy materials provided by TheWell Bioscience Inc..
Let's Design Your 3D Strategy Together
Contact our experts for personalised guidance on selecting the right VitroPrime™ solutions to match your 3D cell culture needs and research goals.