Lipi-Probes & Lipid Droplet Assay Kits for Advanced Lipid Research
Lipid droplets play a central role in lipid metabolism, autophagy and cellular senescence, making them increasingly important targets in cancer, metabolic disease and infection research. Yet, obtaining reliable and high-contrast visualisation of intracellular lipid accumulation remains challenging with conventional staining and quantification methods.
Dojindo’s lipid droplet solutions provide researchers with fast, sensitive and reproducible tools for imaging and quantifying lipid droplets in both live and fixed cells.
Key Challenges
Studying lipid droplet biology often involves balancing sensitivity, specificity and workflow efficiency. Traditional assays may suffer from:
- High cytoplasmic background
- Complex washing procedures
- Limited compatibility with live-cell imaging
- Low reproducibility in quantitative workflows
- Restricted multiplexing possibilities
These limitations can compromise image quality, experimental consistency and throughput.
The Solutions
A range of fluorescent probes specifically designed for rapid and selective lipid droplet staining in live or fixed cells, with minimal background and no washing steps required.
Sensitive fluorimetric assays optimised for plate readers and flow cytometry, enabling scalable and quantitative lipid droplet analysis.
Key Benefits
- High-contrast lipid droplet visualisation with minimal background
- Rapid staining workflow with no washing steps required
- Compatible with both live and fixed cells
- Real-time monitoring of lipid droplet dynamics and metabolism
- Multiplex-friendly fluorescent options for co-staining application
Figure 1: Lipi-Probes Staining of Lipid Droplets. Cells incubated with oleic acid followed by Lipi-probe staining demonstrate rapid and selective visualisation of intracellular lipid accumulation with strong fluorescence intensity.
Overview
| Name | Reference | Application | Highlight | Wavelength |
|---|---|---|---|---|
| Lipi-Blue | LD01-10 | Lipid Droplet Staining |
Immuno- fluorescence |
Ex. 405 nm / Em. 450 – 500 nm |
| Lipi-Green | LD02-10 | Lipid Droplet Staining |
Immuno- fluorescence |
Ex. 488 nm / Em. 500 – 550 nm |
| Lipi-Red | LD03-10 | Lipid Droplet Staining |
Immuno- fluorescence |
Ex. 561 nm / Em. 565 – 650 nm |
| Lipi-Deep Red | LD04-10 | Lipid Droplet Staining |
Immuno- fluorescence |
Ex. 640 nm / Em. 650 – 700 nm |
Why Researchers Chose This Solution
Lipi-Probes enable fast and selective visualisation of intracellular lipid droplets with minimal background and no washing steps required. Compatible with both live and fixed cells, they support real-time monitoring of lipid metabolism and lipid droplet dynamics while simplifying imaging workflows and quantitative analysis.
Key Benefits
- Sensitive and reproducible quantification of intracellular lipid droplets
- Compatible with plate readers, flow cytometry and high-content screening
- Low background fluorescence for reliable data generation
- Live-cell compatible for dynamic lipid metabolism studies
- Fast and scalable workflow suitable for routine and high-throughput analysis
Figure 2: Quantification of Lipid Droplets Using Lipid Droplet Assay Kit, Blue (LD05-10). Lipid droplets are composed of neutral lipids surrounded by phospholipid monolayers and function as specialised intracellular lipid storage compartments.
Figure 3: Advantages of Fluorimetric Lipid Droplet Assays over Colorimetric Methods. Comparison highlighting the superior sensitivity, lower background and improved quantitative performance of fluorimetric assays.
Overview
Why Researchers Chose This Solution
Typical Applications
- Lipid metabolism research
- Cancer biology
- Hepatic steatosis studies
- Autophagy research
- Cellular senescence analysis
- Drug screening and high-content imaging
- Flow cytometry-based lipid quantification
Quick Takeaway
A complete portfolio of lipid droplet imaging and quantification solutions designed to help researchers achieve fast, sensitive and reproducible lipid analysis workflows in live and fixed cells.
Ready to Advance Your Research?
Request a quote or contact Tebubio to discuss the most suitable lipid droplet solution for your research workflow.
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References
Article content created by Tebubio using courtesy materials provided by Dojindo Laboratories .