Bioactive Small Molecule for Stem Cell Biology: When Quality Meets Expectations
In long lasting partnership with Focus Biomolecules and TargetMol we created a tailored and comprehensive offer with a huge variety of factors for your experimental approaches in Stem Cell Biology, such as…
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Our factors meet the highest quality standards concerning to serve your requirements
- Purity
- Specificity
- and biological activity
and come with Certificates of Analysis (CoA) displaying the properties which have been proven in extensive Quality Controls.
We present here a selection of factors directly related to Signaling pathway related to Stem Cell Biology:
As highly conserved enzymes Casein kinases catalyzes serine/threonine phosphorylation of adjacent acidic amino acid residues in the peptide chain. Inhibition of these enzymes can induce reprogramming processes.
Gamma secretases are a multi-subunit protease complexes, integral membrane proteins, that cleave single-pass transmembrane proteins at residues within the transmembrane domain. Modulation of these complexes can influence e.g. neuronal differentiation processes.
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DAPT (LY-374973): γ-secretase inhibitor inhibiting total Aβ and Aβ42 (IC50=115/200 nM). It also inhibits Notch. DAPT induces cell differentiation, autophagy and apoptosis. |
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The Hippo signaling pathway (Salvador-Warts-Hippo [SWH] pathway), is in charge of controlling organ size in animals through regulating cell proliferation and apoptosis. Several modulators of this pathway are involved in cell differentiation processes.
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PY-60 (2765218-56-0) activates YAP transcriptional activity (EC50 = 1.6 µM) via delocalization of annexin A2 (ANXA2) from cell membranes leading to Hippo inactivation, a key controller of YAP. It was able to induce serum-free expansion of MCF10A cells and epidermal keratinocytes and their precursors ex vivo and in adult mice. PY-60 promoted regenerative repair of cutaneous wounds in pig and human models by enabling a pro-proliferative transcriptional program in keratinocytes and dermal cells that accelerated re-epithelization and regranulation of the wound bed. |
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Wnt protein is palmitoacylated by Porcupine (PORCN), which is an O-acyltransferase. PORCN is highly specific to the Wnt signaling pathway and is one of the important targets for WNT-associated cancers.
Wnt protein is palmitoacylated by Porcupine (PORCN), which is an O-acyltransferase. PORCN is highly specific to the Wnt signaling pathway and is one of the important targets for WNT-associated cancers.
STAT proteins are a unique DNA binding family of protein which contain SH2 and SH3 domains binding to peptides containing phosphorylated tyrosine. In the phosphorylated form STAT polymerizes into an activated transcription activator as a homologous or heterodimer. In the nucleus it binds to specific sites of the target gene promoter sequence to promote its transcription.
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Z-Stat (NSC37044) is specific and atypical inhibitor of PKC-ζ with an IC50 of 5 μM. The compound inhibits proliferation and can induce apoptosis in melanoma cell lines. |
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Stemness kinases represent a family of enzymes incl. multiple serine-threonine stemness kinases, such as Nanog. Cancer stemness is referring to the stem-cell-like phenotype of cancer cells. Cancer stem cells the capability of self-renewal by dividing as well as to give rise to all cell types within a tumor; thus they are seen as tumor-initiating cells.
The Wnt signaling pathways represents a group of signal transduction pathways which start by passing signals into a cell through cell surface receptors. Modulating these signals can lead to diverse cell differentiation processes.
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IWP-2 inhibits Wnt secretion and processing. It is able to induce cardiomyocyte differentiation from human pluripotent stem cells. |
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Glycogen synthase kinase 3 (GSK-32) belongs to the family of serine/threonine protein kinases. GSK-3 plays a role quite a number of diseases, including Cancer, Type II diabetes, Inflammation processes, Alzheimer's disease, and bipolar disorder.
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CHIR-99021 potently and selectiveyl inhibits glycogen synthase kinase-3β and GSK3α thus inducing osteoblastogenesis, increasing mineralization and inhibiting adipogenesis in ST2 cells. CHIR-99021 is also able to drive differentiation and reduce proliferation of adult human olfactory epithelium neural precursors. |
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The Hedgehog signaling pathway transmits information to embryonic cells which are necessary for healthy cell differentiation. Malfunction of this pathway can result e.g. in basal cell carcinoma. The pathway also plays a role in cancer stem cell survival.
The JAK-STAT pathway is involved in processes such as cell division, immunity, cell death and tumour formation. JAKs contains a FERM domain, an SH2-related domain, moreover a kinase domain, and finally a pseudokinase domain. The kinase activity allows JAKs to phosphorylate target proteins. Modulating JAK pathway can impact the maintenance of pluripotency and direct cell differentiation processes.
ROCK is a kinase belonging to the AGC serine-threonine kinases. ROCK (Rho-associated proteinKinase) is involved in regulating cell shape and movement by exhibiting its effects on the cytoskeleton. In stem cell biology modulating ROCK can serve as a trigger for cell survival and differentiation.
| Y-27632 is a potent, ATP-competitive inhibitor of ROCK-I and ROCK-II. Y-27632 inhibits isolation-induced apoptosis in mouse prostate stem or progenitor cells. |  |
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| Thiazovivin dramatically improves (200-fold) the efficiency of induced pluripotent stem cell generation from human fibroblasts. The compound directs the conversion of porcine embryonic fibroblasts into adipocytes. |  |
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Smoothened (Smo) is in charge of transducing the -> Hedgehog signal through the cell membrane, which can function through major Hedgehog molecules as Hedgehog ligands, PTCH, Smo, and GLI (canonical pathway) or through the activation of Smo or GLI through other pathways (noncanonical pathway). Activated Smo transduces the signal to the GLI proteins (which represent a family of transcription factors) to finally regulate numerous gene products involved in cell differentiation.
The TGFß1 pathway is involved in a variety of cellular processes in adult organism and in the development of embryos, amongst them cell differentiation, cell growth, apoptosis, cellular homeostasis and other functions. The pathway finally leads R-SMAD/coSMAD complexes accumulation in the nucleus where they act in transcription regulation to direct target gene expression.
| A 83-01 (ALK5 Inhibitor IV) inhibits TGF-β type I receptors ALK5, ALK4, and ALK7. A 83-01 can thus promote reprogramming of mouse fibroblasts into iPSCs. A 83-01 is also widely used in organoid culture. |  |
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| Dorsomorphin acts as a selective and ATP-competitive inhibitor of AMPK while it shows no significant inhibitory effect on ZAPK, SYK, PKCT, PKA and JAK3. Furthermore, bone morphogenetic protein (BMP) type I receptors like ALK2, ALK3 and ALK6 are inhibited. Promotion of cardiomyogenesis in mouse embryonic stem cells in vitro by Dorsomorphin has been reported. |  |
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YAP functions as a transcription co-activator in the Hippo tumor suppressor pathway and controls cell growth, tissue homeostasis and organ size. It induces gene expression through its effect on the TEAD family of transcription factors. YAP can play a complex role in human cancer is complex. In some cases, e.g. in hematological cancers, it can function as a tumor suppressor by inducing apoptosis following to DNA damage.
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