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Efficient enrichment is often the critical step in PTM analysis. Cytoskeleton affinity beads have been optimized to selectively capture modified proteins prior to downstream detection.

Phosphotyrosine Affinity Beads

Ubiquitination Affinity Beads

Acetyl-Lysine Affinity Beads

Figure 1: Measuring total acetylation levels in cell and tissue extracts (Cat. # AAC04-beads)
AAC02-Beads, AAC03-Beads, and AAC04-Beads (50µl bead slurry) were used to IP acetylated proteins from Cos-7 cells either treated (+) or untreated (-) with deacetylase inhibitors [TSA (1µM) and nicotinamide (1mM)] for 6 hours. The total profile of enriched acetylated proteins were eluted and analyzed by western blot with an AAC03-HRP antibody (1:3000). Mouse IgG beads are used as a control for non-specific binding (Cat # CIG02). Each IP assay utilized 1 mg of Cos-7 lysate.

Figure 2: Total SUMO1 profiles detected with the SUMO1 affinity beads (Cat. # ASM11-beads)
HAP1 wildtype (WT) or HAP1-SUMO1-knockout (KO) lysate, was obtained using BlastR lysis and filter system present in the detection kit (Cat. # BK165). 1 mg of each clarified lysate were incubated with 40 µg of SUMO1 affinity reagent; Cat. # ASM11-beads. Bead bound samples were separated by SDS-PAGE and transferred to a PVDF membrane. Enriched SUMO1 samples were analyzed by western blot using anti-SUMO1 antibody at 1:5000 (Cat. # ASM01), and mouse Trubelot Ultra-HRP secondary at 1:1000 in 5% milk.

SUMO Affinity Beads