High-quality mRNAs for robust and reproducible protein expression
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At Tebubio, we understand that reproducibility, speed, and confidence in your reagents are critical to your research. That is why we partner with TriLink BioTechnologies, a pioneer in RNA synthesis, to provide ready-to-use, high-performance premade mRNAs designed to accelerate your discovery programmes, from basic research to advanced therapeutic development.
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Discover how CleanCap® technology improves mRNA performance
TriLink’s premade mRNAs are manufactured using proprietary CleanScript™ in vitro transcription (IVT) or the CleanCap® M6 co-transcriptional capping technology, significantly reducing double-stranded RNA (dsRNA) contaminants while maximising in vivo protein expression.
Manufactured for confidence, consistency and performance
All premade mRNAs are produced under strict quality standards and purified through DNase treatment, diafiltration, and oligo-dT capture. This process enriches full-length transcripts while efficiently removing impurities and salts.
Each product is delivered with a Certificate of Analysis (CoA) including:
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Concentration determination by UV-Vis spectroscopy
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Construct integrity assessed by gel and capillary electrophoresis
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Capping efficiency measured by capping assay
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dsRNA level quantified by dot blot
This ensures reliable performance and peace of mind when moving from optimisation to functional studies.
Quick selection guide: mRNA design options
TriLink offers premade mRNAs in three optimised designs: CleanCap® mRNAs, CleanCap® mRNAs (5moU), and CleanCap® M6 mRNAs (N1MeΨ).
All constructs include:
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A 5′ Cap 1 structure for enhanced stability and translation
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A synthetic 5′ UTR with a strong Kozak sequence
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A mouse α-globin-derived 3′ UTR
Poly(A) tail optimisation
TriLink’s proprietary ModTail™ poly(A) tail modification increases mRNA stability, improves expression consistency, and extends the duration of protein expression—key advantages for next-generation RNA research.
How to choose the optimal design
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ModTail™ → Improved stability and expression consistency
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5moU → Reduced innate immune activation with strong expression
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N1MeΨ → Maximum protein expression with minimal immunogenicity
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5mC → Additional immune suppression and stability
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CleanCap® AG → Robust expression for standard research applications
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CleanCap® M6 → Highest expression levels with the lowest innate immune activation
1. Reporter mRNA
Used to monitor transfection efficiency, protein expression, and assay performance.
| Product Description | Reference |
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| CleanCap® M6 EGFP mRNA / M6 EGFP ModTail™ mRNA (N1MeΨ) | L-8101 / L-8101A |
| CleanCap® EGFP mRNA | L-7601 |
| CleanCap® M6 Firefly Luciferase (FLuc) mRNA / M6 FLuc ModTail™ mRNA (N1MeΨ) | L-8102 / L-8102A |
| CleanCap® Firefly Luciferase (FLuc) mRNA | L-7602 |
| CleanCap® AU EGFP saRNA (5mC) | L-7901 |
| CleanCap® AU EGFP saRNA | L-7801 |
| CleanCap® AU Firefly Luciferase (FLuc) saRNA (5mC) | L-7902 |
| CleanCap® AU Firefly Luciferase (FLuc) saRNA | L-7802 |
| CleanCap® Renilla Luciferase mRNA (5moU) | L-7204 |
Figure 1: ModTail mRNA enhances protein expression and persistence in HEK293T cells
In HEK293T cells, eGFP mRNA, either modified with TriLink’s ModTail™ poly(A) tail or unmodified, was encoded and transfected using CleanCap® M6-capped mRNA. eGFP protein fluorescence was monitored over time using Incucyte®.
Figure 2: Targeted DNA Editing with CRISPR-Cas9
2. Genome editing / functional protein mRNA
Encodes proteins for genome manipulation and targeted cellular functions.
| Product Description | Reference |
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| CleanCap® M6 Cas9 mRNA / M6 Cas9 ModTail™ mRNA (N1MeΨ) | L-8106 / L-8106A |
| CleanCap® Cas9 mRNA / Cas9 mRNA (5moU) | L-7606 / L-7206 |
| CleanCap® Cas9 Nickase mRNA (5moU) | L-7207 |
| CleanCap® M6 Cre mRNA (N1MeΨ) / Cre mRNA (5moU) | L-8111 / L-7211 |
3. Gene replacement / therapeutic target mRNA
Used in phenotypic studies, pathway validation, and protein expression research.
| Product Description | Reference |
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| CleanCap® M6 EPO mRNA (N1MeΨ) | L-8109 |
| CleanCap® EPO mRNA (5moU) | L-7209 |
| CleanCap® β-galactosidase mRNA / β-gal mRNA (5moU) | L-7608 / L-7208 |
5. Self-amplifying RNA (saRNA)
Self-replicating RNA constructs for enhanced and prolonged protein expression.
Figure 3: One SpCas9 and one SaCas9 gRNA ligated 16 times to demonstrate reproducibility of the ligation process. Each dot represents an average of triplicates (48 data points total).
Custom mRNA Manufacturing
Our partner, TriLink, can manufacture our catalogue mRNA with other modified nucleotides and/or other capping technologies, within 2-3 weeks.
Ready to Advance Your Research?
Let our scientific team help you integrate the right RNA tools into your workflow.
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References
Article content created by Tebubio using courtesy materials provided by TriLink Biotechnologies.
Need Expert Guidance for Your mRNA Research?
Our scientific team at Tebubio can help you select the most suitable premade or self-amplifying mRNAs for your experiments, whether in reporter assays, genome editing, protein expression, or immunology studies.
Receive personalised advice to optimise your workflows, enhance reproducibility, and accelerate your discoveries, ensuring reliable performance while minimising dsRNA levels and maximising protein expression.