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What is this webinar about?

Accurate quantification of cytokines and interferons is essential for interpreting immune responses in both research and translational settings. However, low endogenous levels, molecular heterogeneity, and matrix-driven interference can complicate data generation and biological interpretation.

In this session, Dr. Alok Pandey discusses key scientific considerations for reliable cytokine measurement, including subtype diversity in interferons, distinguishing free versus complexed cytokines such as IL-15/IL-15Rα, and evaluating assay performance in disease-state versus healthy matrices. We will walk through real-world data examples to highlight how assay design, reagent quality, and matrix tolerance influence sensitivity, reproducibility, and biological relevance.

The webinar was intended for immunology and translational researchers seeking practical guidance for selecting and interpreting high-sensitivity immunoassays to support confident decision-making in complex biological studies.

Webinar summary

  • Scientific challenges:

    • Low endogenous cytokine levels

    • Molecular heterogeneity across cytokine families

    • Matrix-driven interference impacting assay accuracy

  • Key discussion points by Dr. Alok Pandey:

    • Diversity and functional nuances of interferon subtypes

    • Differentiating free vs. complexed cytokines (e.g., IL-15/IL-15Rα)

    • Assessing assay performance in disease-state vs. healthy matrices

  • Real-world data insights:
    Review case examples demonstrating how assay design, reagent quality, and matrix tolerance affect:

    • Sensitivity

    • Reproducibility

    • Biological relevance

  • Q&A session with scientific expert

Q&A of the live session

  • Why do different assays sometimes give different results for the same cytokine?

    You have to understand your target, and you have to design your assay for that target.

  • What are the main reasons some assays failed to detect endogenous cytokines in disease samples?

    Disease samples are highly complex; they may contain proteins and interfering antibodies that can affect your assay. This makes the analysis challenging. When developing your assay, you must ensure it can account for these factors. You may need to adjust the components to minimise artefacts so that, ultimately, you can accurately measure your target.

  • Why do IL-15 & IL-22 require such assay design?

    For IL-15, the total form (mostly bound) is active, whereas for IL-22, the active form is free; consequently, their assays are fundamentally different and opposite.

Product Resource

If you want to learn more about the product features covered in this webinar, take a moment to visit the PBL products page for more interesting insights.

Your webinar presenters


Alok Pandey, PhD

PBL Vice President


Xavier Warnet, PhD

Project Manager


Afag Asgarova, PhD

Product Manager

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