NAM-Driven 3D Cell Culture Innovation Part. 3

Recreate the Native Cellular Environment
with TheWell Bioscience

TheWell Bioscience offers a comprehensive, xeno-free 3D biomimicking platform designed to support advanced biomedical research, precision medicine, cell therapy and biomanufacturing applications.

VitroGel® hydrogels provide a versatile, xeno-free extracellular matrix environment that supports the growth and maintenance of organoids, spheroids and engineered tissues. Their biofunctional design helps researchers better replicate in vivo conditions while maintaining experimental control.

For researchers developing complex tissue structures, VitroINK® bioinks offer precise printability and structural stability, enabling reproducible tissue fabrication and advanced bioprinting workflows.

RocketCell™ media and reagents simplify cell expansion workflows and prepare cells for successful transition into 3D culture systems, helping laboratories improve efficiency without compromising quality.

VitroPrime™ culture plates feature advanced surface engineering to support more consistent cell attachment, growth and transition between traditional and 3D culture approaches.

Generating meaningful data from complex 3D models can be challenging. Cyto3D® tools help standardise analysis workflows, enabling more quantitative and reproducible biological insights.

Recovering cells from hydrogels without damaging delicate structures is often a critical step. VitroGel® Recovery Solution enables non-enzymatic harvesting of cells and organoids, preserving viability and functionality for downstream applications such as molecular analysis, imaging and functional assays.

CytoGrow™ products provide researchers with defined supplements and growth factors designed to support controlled cell expansion and differentiation while reducing variability.

Improve Growth Factor Delivery with StemCultures

StemCultures addresses this challenge through proprietary microencapsulation technology that delivers sustained growth factor release, better mimicking the native biological niche. Their solutions support a wide range of applications, including stem cell culture, organoid development and primary tumour cell research.

StemBeads®: Sustained Growth Factor Release

StemBeads® encapsulate growth factors within a PLGA hydrogel matrix, protecting protein stability while releasing them gradually over several days.

By maintaining more consistent growth factor concentrations, researchers can:

• Reduce spontaneous differentiation
• Enhance pluripotency maintenance
• Minimise feeding frequency
• Improve experimental consistency

In many workflows, media changes can be reduced to only two or three times per week, saving valuable time and resources.

DISC™ Devices: Long-Term Growth Factor Control

DISC™ Devices combine biocompatible, non-degradable hydrogels with embedded StemBeads® technology.

A single DISC™ continuously releases growth factors at optimal concentrations for one to two weeks while remaining in the culture well throughout the experiment. This approach significantly reduces handling requirements and is particularly valuable for applications requiring precise growth factor withdrawal at defined stages, such as human pluripotent stem cell (hPSC) culture.

Watch the instructional video: FGF2 DISC™ Devices from StemCultures.

Replace FBS with Human-Derived Alternatives

The Belgian Red Cross is a leading European humanitarian organisation involved in the collection and processing of blood-derived products. Its Human Platelet Lysate (hPL) provides a human-derived supplement for the expansion of human cells in research and cell therapy applications.

Compared with traditional FBS supplementation, hPL offers several advantages:

• Faster cell expansion
• Reduced need for additional supplements
• Human-derived composition
• Support for animal-free research strategies

These benefits make hPL an attractive solution for researchers seeking greater physiological relevance while aligning with evolving regulatory and ethical expectations.

Enhance Cell Health and Recovery with 3D Bio-Tissues

Founded as a spin-out from Newcastle University, 3D Bio-Tissues develops innovative bio-inspired technologies that support complex tissue generation for both biomedical and cellular agriculture applications.

CytoBoost™ Revive: Maximise Post-Thaw Recovery

Cryopreservation often results in substantial cell loss, particularly for sensitive cell types such as iPSCs, organoids and primary cultures.

CytoBoost™ Revive helps overcome this challenge by dramatically improving post-thaw recovery, delivering:

• Up to four times more viable cells after thawing
• Improved attachment and survival
• Faster functional recovery
• Reduced risk of failed experiments

For researchers working with valuable or difficult-to-obtain samples, CytoBoost™ Revive provides greater confidence and reproducibility throughout the recovery process.

CytoBoost™: Supporting Cellular Performance

CytoBoost™ acts as a premium support system for cells by enhancing the biochemical and biophysical environment surrounding them.

Unlike conventional supplements that rely on additional growth factors, CytoBoost™ uses carefully selected animal-free, chemically defined macromolecules to improve the effectiveness of culture media components, including:

• Growth factors
• Nutrients
• Proteins
• Enzymes
• Carrier molecules

The result is improved cell health, enhanced culture performance and more robust experimental outcomes.

Build Better 3D Models

From organoid development to stem cell expansion and tissue engineering, Tebubio provides innovative tools that help improve physiological relevance, reproducibility and workflow efficiency.

Ready to Advance Your Research?

Let our scientific team help you integrate the right 3D culture tools into your workflow.

• References

  Courtesy of TheWell Bioscience, StemCultures, Belgian Red Cross & 3D Bio-Tissues Limited .
  

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