Why it Matters
Reliable measurement of antigen-specific T cell responses depends on two critical steps: consistent antigen stimulation and sensitive cytokine detection.
Variability in either step can affect data quality and reproducibility, making standardized reagents essential for immune monitoring studies.
Key Challenges
Obtaining reproducible ELISpot results can be difficult due to:
- Variable antigen preparations
- Inconsistent T cell stimulation
- High background signal
- Limited sensitivity for low-frequency immune responses
The Solution
Combine ImmuneSelect™ Peptide Pools with ImmuneSelect™ ELISpot Kits from Virax Biolabs to create a standardized workflow from antigen stimulation through cytokine detection.
Together, they provide reliable functional immune monitoring while helping reduce experimental variability.
Most Competitively Priced Human Glutamatergic Neuron Models On The Market!
Key Benefits
- Standardized antigen stimulation for consistent T cell activation
- Sensitive detection of low-frequency cytokine-secreting cells
- Single-cell resolution for accurate immune response quantification
- Reduced background with clear spot definition
- Optimized for PBMC-based immune monitoring assays
| Product | Description | Product Link |
|---|---|---|
|
Wild-Type ioGlutamatergic Neurons
|
Ready-to-use human iPSC-derived glutamatergic neurons with rapid maturation, high purity and proven reproducibility. | View Product → |
|
MAPT S305N Disease Model
|
Human glutamatergic neurons carrying the MAPT S305N mutation for frontotemporal dementia and tauopathy research. | View Product → |
|
CRISPR-Ready ioGlutamatergic Neurons
|
Ready-to-use neurons engineered for rapid CRISPR knockout, activation or interference studies without stable Cas9 cell lines. |
CRISPR Knockout →
CRISPR Activation → CRISPR Interference → |
ImmuneSelect™ ELISpot Kits
ImmuneSelect™ Peptide Pools
Key Features
- Detect low-frequency antigen-specific immune responses
- Single-cell cytokine quantification
- Low background and high spot clarity
- Compatible with primary cells, including PBMC assays
Key Features
- Synthetic peptides for improved lot-to-lot consistency
- Water-soluble formulation for easy handling
- Optimized for PBMC stimulation
- Reduced variability compared with biological antigen preparations
Figure 1. ImmuneSelect ELISpot kits capture the immune system in action.
By stimulating PBMCs with infection-specific peptides, a targeted T cell response is initiated and is visualized directly on the plate surface. As T cells secrete cytokines in response to stimulation, the cytokines are captured by antibodies around the cell on the surface of the well and visualised via a colorimetric reaction. Each cytokine-secreting cell forms a distinct "spot", creating a visual footprint that provides a precise count of responding cells.
Why Choose ImmuneSelect™?
ImmuneSelect™ Peptide Pools and ImmuneSelect™ ELISpot Kits provide a complementary solution for standardized functional immune monitoring.
By combining reproducible antigen stimulation with sensitive cytokine detection, researchers can generate consistent, quantitative T cell response data across a wide range of immunology applications.
Case Study
ImmuneSelect™ Peptide Pools targeting Respiratory Syncytial Virus (RSV) and Cytomegalovirus (CMV) were used to stimulate human PBMCs, followed by analysis using the ImmuneSelect™ Human IFN-γ ELISpot Assay.
The combination produced enhanced IFN-γ spot formation, demonstrating efficient activation and detection of virus-specific T cell responses.
This workflow supports reliable characterization of antigen-specific immunity while avoiding the variability associated with live pathogens or biological antigen preparations.
Figure 2. ImmuneSelect ELISpot analysis of IFN-γ secreting cells (IFN-γ spot forming units (SFU)) following stimulation of PBMCs with pathogen-specific peptide pools (CMV and RSV). [n=3]
Figure 3. Representative images of ImmuneSelect ELISpot wells showing PBMCs stimulated with pathogen-specific peptide pools compared to positive control wells (CD3/CD28/CD2 cocktail) and unstimulated wells
Combining standardized peptide stimulation with sensitive ELISpot-based cytokine detection enables reliable characterization of antigen-specific T cell responses across infectious disease, vaccine and immunology research workflows.
Looking for the right ELISpot assay or peptide pool for your research?
Contact our team for help selecting the best ImmuneSelect™ solution for your application.
-
References
Article content created by Tebubio using courtesy materials provided by Virax Biolabs.