From RNA delivery to biological activity
RNA therapeutics need to be evaluated beyond delivery and expression. Tebubio provides flexible in vitro RNA testing and cell-based assays to assess RNA biological activity and mechanism of action.
From standardized assays to customized studies, we work with relevant 2D and 3D cellular models, including primary and immune cells, to help characterize your RNA candidates and delivery systems.
Build the right validation workflow
RNA projects often require several complementary measurements rather than a single assay.
This integrated approach helps connect RNA delivery with functional biological outcomes and provides a more complete characterization of your candidate.
Choose the right cellular model
The right model is essential to generate meaningful biological data.
Tebubio provides access to 750+ cell lines, together with primary and immune cells and 3D cellular models.
Sequence Optimization
- Plasmid and linear DNA
- Templates for mRNA, saRNA, circRNA and polycistronic RNA
- Enhanced protein expression Reduced immunogenicity Minimized truncated products
- 100 µg to 10 mg, up to 10 kb
- Choice of capping, modified and fluorescent nucleotides
- Various purification methods and quality controls
- Chemical transfection or LNP
- Encapsulation from 10 µg to 5 mg RNA
- Large choice of lipids, including conjugated
- Cell lines and primary cells 2D and 3D
- Uptake, delivery, expression
- Cellular response Functional activity Inflammation
- Plasmid and linear DNA
- Templates for mRNA, saRNA, circRNA and polycistronic RNA
- Enhanced protein expression Reduced immunogenicity Minimized truncated products
- 100 µg to 10 mg, up to 10 kb
- Choice of capping, modified and fluorescent nucleotides
- Various purification methods and quality controls
- Chemical transfection or LNP
- Encapsulation from 10 µg to 5 mg RNA
- Large choice of lipids, including conjugated
- Cell lines and primary cells 2D and 3D
- Uptake, delivery, expression
- Cellular response Functional activity Inflammation
Fig 1. Tracking of eGFP mRNA delivered with Cy3-LNP in spheroids of colorectal cancer cells.
Assess RNA delivery and expression
Customize your RNA according to your research needs, including 5′ capping, modified nucleotide incorporation, poly(A) tailing, and fluorescent labeling.
Understand whether your RNA reaches the cell, is delivered effectively and produces the expected protein.
Our approaches can assess:
Fig 2. Live cell imaging of the expression of eGFP-Cy5 mRNA in HEK293 cells over 72h.
Depending on the study, we combine fluorescence imaging with complementary techniques such as ELISA, Western blot and RT-qPCR.
Reporter systems and fluorescently labelled RNA and lipids can also be combined to distinguish delivery, uptake and protein expression within the same cellular model.
Validate biological activity
Expression alone does not demonstrate that an RNA candidate is biologically active.
Tebubio develops and performs cell-based functional assays to measure the biological response to your RNA candidate and investigate its mechanism of action.
Depending on the therapeutic and cellular model, readouts can include:
Fig 3. Cytotoxicity of an eGFP-tagged mRNA in spheroids of colorectal cancer cells.
Biomarker and pathway analysis can provide additional insight into the biological mechanisms triggered by your RNA candidate.
From RNA to biological validation
Whether you're developing vaccines, oncology therapeutics or protein replacement strategies, Tebubio supports every stage of your workflow, from custom saRNA production to delivery and biological validation.
Ready to advance your RNA project?
Why Choose Tebubio as Your Laboratory Service Provider
EXPERTISE
PhD-level expertise
Scientific guidance tailored to your research question.
FLEXIBILITY
Support at the right scale
From a single assay to a complete study, with solutions that evolve with your project.
SIMPLICITY
One partner, less complexity
From study design to results, with a dedicated project manager by your side.
ACCESSIBILITY
European expertise, global reach
Your research, handled in France, with close collaboration and support for researchers in Europe.
Scientific Resources
Explore how to optimize Lipid Nanoparticules for mRNA delivery for drug development.
This study highlights key formulation parameters: buffer composition, lipid selection; crucial for enhancing transfection and therapeutic efficacy.
Xavier Warnet PhD, our CRO's project manager published a review on how scientists have developed lipid nanoparticles (LNPs) that make it possible to deliver RNA in a more precise manner, paving the way for future development in oncology.
RNA Therapeutics
Cell Therapy
Biotherapeutic Discovery​
Choose Tebubio as Your Laboratory Service Provider
EXPERTISE
PhD-level expertise
Scientific guidance tailored to your research question.
FLEXIBILITY
Support at the right scale
From a single assay to a complete study, with solutions that evolve with your project.
SIMPLICITY
One partner, less complexity
From study design to results, with a dedicated project manager by your side.
ACCESSIBILITY
European expertise, global reach
Your research, handled in France, with close collaboration and support for researchers in Europe.