Generate Functional NK Cells for Next-Generation Immunotherapy Research

Natural Killer (NK) cells are rapidly emerging as a key pillar of next-generation immunotherapies. The BPS Bioscience NK Cell Expansion Kit (78927) enables researchers to efficiently generate large quantities of functional NK cells for downstream applications.

NK cell therapies are gaining momentum as safer, off-the-shelf alternatives to CAR-T.  A major bottleneck in research is obtaining sufficient, high-quality NK cells. This kit provides a robust, reproducible solution for NK cell expansion.

Key Features

• High expansion efficiency
  → Achieve significant NK cell proliferation from PBMCs
• Physiologically relevant activation
  → Uses engineered feeder cells to promote functional NK profiles
• Ready-to-use workflow
  → Simplifies a complex and time-consuming process
• Reproducible results
  → Standardized conditions for reliable outcomes

Applications

• NK cell therapy development (CAR-NK, adoptive cell therapy)
• Immuno-oncology research
• Cytotoxicity and functional assays
• Drug screening and validation

Figure: Flow cytometry analysis of the cytotoxicity profile of expanded NK cells. Human PBMCs (A) and isolated NK cells (B) were expanded ex vivo for 14 days. Expanded NK cells were co-cultured with CFSE-labeled K562 target cells at different Effector:Target (E:T) cell ratios at 37°C for 4 hours. 7-AAD was used to detect NK cell cytotoxicity towards K562 cells by flow cytometry (left). Flow cytometry results were used to generate killing curves (right).

Benefits

• Generate high-quality NK cells in-house
• Accelerate timelines for functional assays and screening
• Reduce variability compared to manual expansion methods

The NK Cell Expansion Kit (78927) is suitable for the ex vivo culture and expansion of human natural killer (NK) cells. It contains engineered Growth-Arrested NK Feeder Cells and NK Cell Basal Medium. The Growth-Arrested NK Feeder Cells are K562 cells engineered to express membrane bound IL-21, and other components, to drive the robust activation and expansion of NK cells. The kit provides enough growth-arrested feeder cells for a starter population of 1 million purified NK cells or PBMC cells.

The NK Cell Expansion Kit (78927) is a powerful, ready-to-use solution to generate functional NK cells and accelerate immunotherapy research workflows.

Why Choose the BPS Bioscience NK Cell Expansion Kit?

Researchers need more than NK cell expansion, they need consistent, functional, and reproducible results.

The BPS Bioscience NK Cell Expansion Kit combines engineered IL-21-expressing feeder cells with optimized media to deliver robust proliferation and reliable generation of high-quality NK cells for CAR-NK development, cytotoxicity assays, and immuno-oncology research.

Quick Takeaway

Expand high-quality, functional human NK cells with confidence:

• Robust expansion from PBMCs or purified NK cells
• Standardized, ready-to-use workflow 
• Reproducible results across experiments 
• Supports CAR-NK, immuno-oncology and cytotoxicity studies
• Easily combine with NK cytotoxicity assays for functional validation 

Need Help Building Your NK Cell Workflow?

Whether you need support selecting the right products or require customized assay development and cell-based services, our scientific experts are here to help. Discover how tebubio's CRO capabilities can support your immunotherapy research from cell expansion to functional characterization.

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  Article content created by Tebubio using courtesy materials provided by BPS Bioscience.