DNA Extraction

Working in Oncology, Immunology, Functional Genomics, or Neuroscience?

Tebubio & NZYtech are Here to Guide your Research

Follow a complete workflow

 

 

 

What sample types are you working with?

Name Cell and Tissues Plants and Fungi Soil, Stool and Complex
Product Image NZY Total RNA Isolation Kit ✔
NZY DNA/RNA/Protein Isolation Kit ✔ ✔
NZY Plant/Fungi RNA Isolation Kit ✔ ✔
NZY Stool RNA Isolation Kit ✔ ✔ ✔

What features are important for your workflow?

Name Reaction Temperature cDNA Synthesis time Inhibitor Resistance
Supreme NZY RT SuperMix 5x 30 °C - 70 °C 1 - 10 min +++
NZY RT SuperMix 5x 50 °C 10 min ++
Supreme HC NZY First-Strand Flexible Pack 42 °C - 70 °C 1 - 10 min +++
NZY First-Strand cDNA Synthesis kit 50 °C - 55 °C 10 min +
NZY First-Strand Flexible Pack 50 °C - 55 °C 10 min +

Achieve full-length cDNA synthesis in just 1 minute

Tebubio, through NZYtech, offers a new solution for synthesizing high-quality cDNA in a faster, simpler and more efficient way. The Supreme NZY RT SuperMix 5x contains all the necessary components for cDNA synthesis in a single tube, reducing pipetting errors and optimizing the workflow, while maintaining the quality and reliability of the results.

Features Supreme NZY RT SuperMix 5x
Optimal reaction temperature 60ËšC
Reaction time 10–15 min*
Amplification of complex templates High Efficiency
Primers Optimized mixture of Oligo (dT)18 and random hexamers

* Includes all steps prior to cDNA synthesis, such as primer annealing.

Figure 1: With zero annealing time, ultra-fast cDNA synthesis in just 1 minute, and complete RT inactivation in 5 minutes, Supreme NZY RT SuperMix 5x outperforms competitors at every step. 

What features are important for your workflow?

Name UDG/UNG Visual Aid Hot-Start
Product Image NZYSupreme qPCR Green Master Mix (2x) Multiple
NZYSpeedy qPCR Green Master Mix (2x) Multiple
NZYTrace qPCR Green Master Mix 2x ✔ Multiple
NZYTrace qPCR Green Master Mix 2x (UDG) ✔ ✔ Multiple

NZYSupreme qPCR Green Master Mix

NZYSupreme mixes offer ultra-sensitive detection with a multiple hot-start mode and are compatible with a variety of thermocyclers and incorporate passive reference dyes, such as ROX™ dye, to ensure accurate signal normalization (see devices below).

  1. NZYSpeedy qPCR Green Master Mix (2x), ROX plus
  2. NZYSpeedy qPCR Green Master Mix (2x), ROX
  3. NZYSpeedy qPCR Green Master Mix (2x)

Figure 2: Compatible devices with NZYSupreme qPCR Green Master Mix's.

Detection of Large1 from human gDNA Amplification Plot

Figure 3: Benchmark Analysis against 9 providers. A 5-fold serial dilution of human genomic DNA was used, resulting in a high sensitivity and linearity signal confirmed for NZYtech’s master mix in amplifications. A unique peak corresponding to the desired amplicon was also detected with no primer-dimers nor non-specific amplifications during the experiments.

Suitable for Routine Screening Workflows

NZYTrace qPCR Green Master Mix incorporates dUTP together with a thermolabile uracil-DNA glycosylase (UDG) to efficiently remove carryover contamination from uracil-containing amplicons generated in previous runs, helping to prevent false positives in demanding routine workflows. Additionally, it includes a yellow buffer for visual verification of correct pipetting without affecting qPCR performance.

Applications:

  • Gene expression studies, 
  • Pathogen and biomarker detection
  • Amplification of low-copy or otherwise challenging DNA targets. 

Want to Speed your gene expression set up?

Extract your sample RNA and jump directly towards qPCR with One-Step qPCR Kit

ROX™ dye compatibility

NO ROX ROX ROX PLUS
Product Image One-step NZYSpeedy RT-qPCR Green Kit One-step NZYSpeedy Room temperature-qPCR Green kit One-step NZYSpeedy Room temperature-qPCR Green kit, ROX One-step NZYSpeedy Room temperature-qPCR Green kit, ROX plus

Find the full offer for your life science experiments

  • References


    Article content created by Tebubio using courtesy materials provided by NZYtech.

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