Senescent cells

SPiDER Blue Cellular Senescence Detection Kit

Cellular senescence is involved in ageing, cancer, fibrosis and many chronic diseases. Reliable detection of senescent cells is essential for understanding disease mechanisms and evaluating therapeutic interventions. However, traditional SA-β-Gal assays can be difficult to combine with multiplex fluorescence workflows.

Key Challenges

Researchers need a sensitive and reproducible method to detect senescence markers while preserving compatibility with immunofluorescence, co-localisation studies and high-content imaging applications.

Overview

SPiDER Blue Cellular Senescence Detection Kit

A fluorescence-based assay for sensitive detection of senescence-associated β-galactosidase (SA-β-Gal) in fixed cells, enabling straightforward integration into multiplex staining workflows.

 

Figure 1: Detection mechanism of senescent cells by SPiDER Blue (SG07).

Key Benefits

  • Sensitive detection of SA-β-Gal activity in fixed cells
  • Blue fluorescence enables clean multiplexing with green and red fluorophores
  • Compatible with immunofluorescence and antibody-based staining
  • Reduced background signal thanks to the optimised assay buffer
  • Suitable for microscopy, flow cytometry, plate readers and high-content imaging

Key Features

  • Fluorescent detection of SA-β-Gal activity
  • Blue emission spectrum (Ex 350–450 nm / Em 400–500 nm)
  • Compatible with multiplex fluorescence assays
  • Designed for fixed-cell applications

Typical Applications

  • Cellular senescence research
  • Immunofluorescence studies
  • Co-localisation experiments
  • High-content screening
  • Ageing and cancer biology research

Case Study

Researchers investigated lipid droplet accumulation in A549 cells following doxorubicin-induced senescence. Senescent cells were identified using SPiDER Blue, while lipid droplets were detected using Lipi-Deep Red. Learn more about how to visualise lipid droplets here.

The study showed increased lipid droplet accumulation in SA-β-Gal-positive senescent cells, demonstrating the value of SPiDER Blue for multiplex imaging and phenotypic characterisation of senescence-associated cellular changes.

 

Figure 2. Co-staining of A549 cells with SPiDER Blue and Lipi-Deep Red showing lipid droplet accumulation in senescent cells.

Quick takeaway

A sensitive fluorescence-based solution for detecting senescent cells in fixed samples while maintaining full compatibility with multiplex imaging workflows. Contact Tebubio to learn more about SPiDER Blue and discuss your senescence research applications.

Ready to Advance Your Research?

Explore SPiDER Blue and enable sensitive detection of senescent cells in fixed samples, while seamlessly integrating SA-β-Gal analysis into multiplex fluorescence workflows.