Detect Low-Abundance Proteins With High Sensitivity

Many proteins of biological and clinical interest are present at extremely low concentrations, making accurate quantification difficult with conventional immunoassays. As biomarker discovery, immuno-oncology, cell therapy and translational research continue to advance, researchers increasingly require detection methods that combine exceptional sensitivity with reliable specificity.

Key Challenges

Detecting low-abundance proteins often means compromising between sensitivity, reproducibility and sample consumption. Conventional assays may fail to detect subtle biological changes or require larger sample volumes that are not always available.

Researchers need a solution that delivers:

The Solution: NexaTag® - The Next Generation of Ultrasensitive ELISA

NexaTag® is a quantitative immuno-PCR (qIPCR) platform that combines the specificity of a sandwich immunoassay with magnetic bead capture and the amplification power of quantitative PCR.

This innovative approach enables highly sensitive protein detection while maintaining the robustness and ease of use researchers expect from immunoassays.

Figure 1: NexaTag leverages the principles of quantitative immuno-PCR (qIPCR) in a bead-based sandwich assay format.

Looking for special target? 

With more than 6,000 validated assays available, NexaTag® supports a broad range of research applications, including immuno-oncology, inflammation, cell therapy and neuroscience: 

Explore the complete NexaTag® portfolio, featuring assays for thousands of protein targets across multiple research areas and species:

Why Researchers Choose NexaTag®

Detect What Conventional ELISA Misses

• Limits of detection down to femtogram/mL
• Up to 1,000× greater sensitivity than conventional ELISA

Preserve Precious Samples

• Requires as little as 5 μL per replicate
• Ideal for precious or limited-volume samples

Confidence in Every Result

• Dual-recognition sandwich assay minimizes false positives
• Excellent reproducibility for demanding research applications

Simple Integration into Existing Workflows

• Compatible with standard real-time PCR instruments
• Complete workflow in approximately 4 hours

Multiplex and Multi-Species Flexibility

• Analyze up to 5 targets simultaneously
• Available for 16 research species, including human, mouse, rat and non-human primates

Key Technology Features

• Magnetic bead-based target capture
• DNA barcode signal amplification
• Real-time qPCR detection
• Quantitative immuno-PCR (qIPCR) workflow
• Standard qPCR instrument compatibility

Ideal Applications

NexaTag® is particularly well suited for:

• Biomarker discovery
• Immuno-oncology research
• Cell therapy development
• Translational research
• Research requiring ultrasensitive protein detection

Quick Takeaway

NexaTag® combines the specificity of sandwich immunoassays with the sensitivity of qPCR amplification, enabling accurate quantification of ultra-low abundance proteins while using minimal sample volume. It is an ideal solution for researchers seeking highly sensitive, reproducible protein detection without changing their existing qPCR workflow.

Accelerate Your Research with Tebubio

Looking beyond target detection? Our Contract Research Organisation (CRO) supports projects from early discovery through preclinical research with flexible, on-demand services designed to accelerate your research and reduce development timelines.

Talk to our experts to discuss your project, identify the best targets for your study, or discover how our CRO services can support your next research milestone.

• References

  Article content created by Tebubio using courtesy materials provided by Raybiotech.