Save 20% on Proven Tools for Cell Dynamics
A curated selection of actin, microtubule, PTM, and GTPase tools, trusted by cell biology labs worldwide.
Offer valid until February 28, 2026.
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Understanding cytoskeletal dynamics is central to deciphering cell shape, motility, signaling, and fate. Through its partnership with Cytoskeleton, Tebubio is offering an exclusive 20% discount on a selection of best-in-class research tools designed to deliver reproducible, publication-ready data. From F-actin visualization and polymerization assays to microtubule dynamics, post-translational modifications, and small G-protein activity, this curated collection supports both exploratory and hypothesis-driven research, without compromising on quality or workflow efficiency. Explore the eligible products below and equip your laboratory with tools trusted by cytoskeleton experts. |
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F-Actin Visualization & Dynamics: Fluorescent phalloidins, actin polymerization & quantification kits
Post-Translational Modifications (PTMs): Signal-Seeker® kits for phosphorylation, ubiquitination, SUMOylation & acetylation
Microtubules & Tubulin Dynamics: Purified tubulin, labeled tubulin, stabilized microtubules & polymerization assays
Small GTPase Activation & Signaling: G-LISA assays and pull-down activation kits
Additional & Supporting Tools: GEF/GAP assays, lysate prep, effector beads
Acti-Stain™ Fluorescent Phalloidins
Filamentous actin (F-actin) is a polymerized form of actin, composed of adenosine triphosphate-actin monomers assembled into helical filaments with intrinsic polarity. This structure is essential for maintaining cell shape, enabling motility, and facilitating intracellular transport. Phalloidin conjugates are commonly used in fluorescence imaging to selectively stain F-actin across a range of sample types, including fixed cells, tissue sections, and cell-free systems.
Swiss 3T3 cell stained with anti-vinculin (red), Dapi (blue nucleus) and F-actin is stained with Acti-stain™ 488 (green F-actin, Cat.# PHDG1-A).
Actin Polymerization Assay Kit
Actin polymerization is the process by which globular actin (G-actin) monomers assemble into filamentous actin (F-actin), forming dynamic cytoskeletal filaments that are essential for cell shape, motility, and intracellular transport.
Through Cytoskeleton, Tebubio offers a simple, fluorescence-based assay using pyrene-labeled actin to:
- Monitor actin polymerization in vitro via kinetic fluorescence increase
- Characterize of actin-binding proteins
- Screen for actin-binding protein ligands
Post-Translational Modification Tools
PTMs play essential roles in regulating cellular processes including signal transduction, gene expression, cell cycle control, immune responses, and metabolism. With user-friendly kits and streamlined IP and western blot workflows, Tebubio via Cytoskeleton makes endogenous PTM analysis more accessible.
Signal-Seeker® Kits
The Signal-Seeker® PTM detection kit uses affinity beads to enrich PTM proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard Western blot procedures, and the modified protein of interest is detected using a primary antibody.
- BK160: Signal-Seeker® Phosphotyrosine Detection Kit (30 assays)
- BK160-S: Signal-Seeker® Phosphotyrosine Detection Kit (10 assays)
- BK161: Signal-Seeker® Ubiquitin Detection Kit (30 assays)
- BK161-S: Signal-Seeker® Ubiquitin Detection Kit (10 assays)
- BK162: Signal-Seeker® SUMOylation Detection Kit (30 assays)
- BK162-S: Signal-Seeker® SUMOylation Detection Kit (10 assays)
- BK163: Signal-Seeker® Acetyl-Lysine Detection Kit (30 assays)
- BK163-S: Signal-Seeker® Acetyl-Lysine Detection Kit (10 assays)
- BK164: Signal-Seeker® Acetyl-Lysine Detection Kit - AAC03-Beads (30 assays)
- BK165: Signal-Seeker® SUMOylation-1 Detection Kit (30 assays)
- BK165-S: Signal-Seeker® SUMOylation-1 Detection Kit (10 assays)
Microtubules
Microtubules are fundamental components of the cytoskeleton that control essential cellular processes such as cell division, intracellular transport, cell polarity, and the maintenance of cell shape.
They form the mitotic spindle during mitosis, serve as tracks for motor proteins like kinesin and dynein, and play key roles in neuronal structure and function. The building block of a microtubule is a tubulin dimer.
Tebubio is offering labeled and fluorescent tubulins, supporting cutting-edge research in microtubule dynamics, cell division and motility.
Get straight to results with pre-formed microtubules, ready-to-use, high-purity for reliable results in motor assays, drug screens, and MT-binding studies.
Stabilized microtubules have been prepared from tubulin protein purified from porcine brain that is ≥99% pure.
Tubulin Polymerization Assay Kit
This assay measures tubulin polymerization dynamics using a fluorescent reporter that incorporates into microtubules as they form. It is designed to monitor the real-time kinetics of microtubule assembly and disassembly, which is critical for understanding cellular functions and screening pharmacological agents.
Tubulin and Fluorescent Tubulin
Polymerized tubulin labeled with Rhodamine tubulin,
Cat.# TL590M-B.
- T240-A: Tubulin (porcine brain, >99% pure)
- T240-B: Tubulin (porcine brain, >99% pure)
- T240-C: Tubulin (porcine brain, >99% pure)
- T240-D: Tubulin (porcine brain, >99% pure)
- T240-DX: Tubulin (porcine brain, >99% pure)
- TL440M-A: Tubulin (AMCA dye labeled; porcine, micro-injctn)
- TL440M-B: Tubulin (AMCA dye labeled; porcine, micro-injctn)
- TL488M-A: Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn)
- TL488M-B: Tubulin (HiLyte 488TM dye labeled; porcine, micro-injctn)
- TL590M-A: Tubulin (Rhodamine labeled; porcine, micro-injctn)
- TL590M-B: Tubulin (Rhodamine labeled; porcine, micro-injctn)
- TL620M-A: Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn)
- TL620M-B: Tubulin (X-rhodamine labeled; Bovine Brain micr-injctn)
- TL670M-A: Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn)
- TL670M-B: Tubulin (HiLyte 647TM dye labeled; porcine, micro-injctn)
G-LISA Kits
The Ras, Arf, and Rho families mediate a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, membrane trafficking and apoptosis.
The traditional methods of measuring the level of activation of these Small GTPases have been pull-down activation assays. However, these methods are time consuming, require large amount of sample, tend to not be very consistent and are not suitable for plate reader technology.
Therefore, Cytoskeleton has developed G-LISA, surpassing those advantages.
- BK121: RhoA G-LISA Activation Assay (luminescence)
- BK124: RhoA G-LISA Activation Assay (colorimetric)
- BK124-S: RhoA G-LISA Activation Assay (colorimetric)
- BK125: Rac1,2,3 G-LISA Activation Assay Kit (colorimetric)
- BK126: Rac1 G-LISA Activation Assay Kit
- BK127: Cdc42 G-LISA Activation Assay Kit (colorimetric)
- BK127-S: Cdc42 G-LISA Activation Assay Kit (colorimetric)
- BK128: Rac1 G-LISA Activation Assay Kit (colorimetric)
- BK128-S: Rac1 G-LISA Activation Assay Kit (colorimetric)
- BK129: RalA G-LISA Activation Assay Kit (colorimetric)
- BK131: Ras G-LISA Activation Assay Kit (colorimetric)
- BK135: G-LISA Bundle (BK135 = BK124-S + BK127-S + BK128-S) (colorimetric)
- BK150: RhoA ELISA Kit (colorimetric)
Small G-Proteins Biological Activity Assays
Small GTPases alternate between an active (GTP-bound) state, enabling interaction with effectors to drive signaling, and an inactive (GDP-bound) state, unable to bind effectors so halting signal transduction. The following pull-down assay enables:
- Isolating GTP-bound forms using the binding domain of an effector protein immobilized on agarose beads. The bound GTPase is then detected and quantified by Western blotting using a specific antibody.
- Studying Small G-Proteins signaling pathways
Additional Tools
Don't miss the remaining products eligible in this promotion
2.4 DNA Synthesis
| Product Description | Reference | Highlights / Detection |
|---|---|---|
| EdU Cell Proliferation Imaging Assay Kit | Imaging Kit ClickTech EdU | Senescent cells are EdU-negative. Highly photostable click chemistry detection. Detection: In vitro fluorescence microscopy. |
| EdU Cell Proliferation Flow Cytometry Assay Kit | FC Kit ClickTech EdU | Quantitative assessment of DNA synthesis. Detection: In vitro flow cytometry. |
| EdU Cell Proliferation High Throughput Screening | ClickTech EdU | Scalable format for screening applications. Detection: In vitro high-throughput screening. |
| In Vivo EdU Cell Proliferation Assay for Imaging | In Vivo ClickTech EdU | EdU incorporation in vivo. Detection: Fluorescence microscopy. |
| In Vivo EdU Cell Proliferation Assay for Flow Cytometry | In Vivo ClickTech EdU | EdU incorporation in vivo. Detection: Flow cytometry. |
| In Vivo EdU Cell Proliferation Assay for High-throughput Screening | In Vivo ClickTech EdU | EdU incorporation in vivo. Detection: High-throughput screening. |
3. Cell Cycle Arrest
| Product Description | Reference | Highlights / Detection |
|---|---|---|
| THUNDERâ„¢ Phospho-p53 (S15) + Total p53 | THUNDER | TR-FRET-based intracellular quantification. Detection: Adherent & suspension cells. |
| Human p16INK4a ELISA Kit | ELH-CDKN2A | Quantitative measurement of p16INK4a, key senescence marker. Detection: ELISA / Serum, plasma, supernatants. |
| Cell Cycle Assay Solution Deep Red | C548-10 | Determines G0/G1, S and G2/M phase distribution. Detection: Flow cytometry / Live or fixed cells. |
4. Senescence-Associated Secretory Phenotype (SASP)
| Product Description | Reference | Highlights / Detection |
|---|---|---|
| Human IL-6 Kits | IL-6 | IL-6 sustains intracrine senescence signalling. Detection: ELISA / CLIA / ELISpot. |
| Human IL-8 Kits | IL-8 | Key SASP cytokine maintaining senescence. Detection: ELISA / CLIA / ELISpot. |
| Human Inflammation Array | QAH-INF-1 | Multiplex quantitative detection of ≥10 inflammatory mediators. Customisable arrays. Detection: Quantibody® ELISA array. |
| CXCL1 Detection Kit | CXCL1 | Neutrophil recruitment and chronic inflammation marker. Detection: ELISA, TR-FRET, qPCR. |
| CCL2/MCP-1 Detection Kit | CCL2, MCP-1 | Immune cell recruitment and inflammageing driver. Detection: ELISA, TR-FRET, qPCR. |
| Matrix Metalloproteinase (MMP) Detection Kits | MMP-1, MMP-7, MMP-9 | ECM remodelling enzymes upregulated in senescence. Detection: ELISA, TR-FRET, qPCR. |
Biomarker Quantification & Custom Assay Services
Accelerate your senescence research with Tebubio’s biomarker quantification services. Our CRO team supports your projects with tailor-made assay development and execution, from SASP profiling and senescence marker quantification to pathway-focused biomarker mapping to streamline your experimental workflow, reduce development time, and generate robust, reproducible data for your research.
Figure 2. Components of the senescence-associated secretory phenotype (SASP), including cytokines, chemokines, proteases and growth factors
5. Targeting Cellular Senescence and SASP
Targeting cellular senescence is now a central strategy in ageing and disease-related research. Two complementary therapeutic approaches have emerged. Senolytics selectively induce apoptosis in senescent cells, reducing senescent cell burden and improving tissue function. In contrast, senomorphics modulate pathological SASP signalling by inhibiting SASP production or activity without eliminating senescent cells (Figure 2).
These strategies aim to mitigate chronic inflammation, preserve tissue homeostasis and improve resilience to age-associated pathologies.
Senolytics
| Compound | Mechanism / Target |
|---|---|
| 17-AAG | HSP90 inhibitor |
| ABT-263 (Navitoclax) | Bcl-2 family inhibitor |
| ABT-737 | Bcl-2 family inhibitor |
| BPTES | Glutaminase GLS1 inhibitor |
| Cycloastragenol | Telomerase activator |
| Dasatinib | Src kinase inhibitor |
| Enzastaurin | PKC / PI3K-Akt inhibitor |
| JQ1 | BRD4 inhibitor |
Senomorphics
| Senomorphic Compound | Mechanism / Target |
|---|---|
| BAY 11-7082 | IKK inhibitor |
| FK866 HCl | NAMPT inhibitor |
| I-BET762 | BET Bromodomain inhibitor |
| Metformin HCl | AMPK activator |
| ML324 | KDM4 histone demethylase inhibitor |
| Rapamycin | mTOR inhibitor |
| Ruxolitinib | JAK/STAT pathway inhibitor |
| SB 203580 | p38MAP kinase inhibitor |
| SR9009 | REV-ERB agonist |
| SRT1720 HCl | SIRT1 activator |
| Zileuton | 5-LO inhibitor |
Senescence Inducers
| Senescence Inducer | Mechanism / Target |
|---|---|
| 2’,3’-cGAMP | Endogenous STING agonist |
| Abemaciclib | CDK4/6 inhibitor |
| Actinomycin D | Transcription inhibitor |
| Aphidicolin | DNA polymerase inhibitor |
| Decitabine | DNA hypomethylation agent |
| Doxorubicin | DNA damaging agent |
| BIBR1532 | Telomerase inhibitor |
| Bleomycin | DNA double-strand break inducer |
| Daunorubicin HCl | DNA damaging agent |
| Entinostat | HDAC inhibitor |
| Etoposide | Topoisomerase II inhibitor |
| Ingenol-3-angelate | PKC activator |
| JPH203 | LAT1 inhibitor |
| Mitomycin C | DNA damaging agent |
| Nutlin-3 | MDM2 antagonist |
| OSS-128167 | SIRT6 inhibitor |
| PMA | PKC activator |
| Ribociclib | CDK4/6 inhibitor |
| RITA | p53 activator |
| SN-38 | Topoisomerase I inhibitor |
| Temozolomide | Autophagy inducer |
| Tenovin-1 | SIRT1/2 inhibitor |
| WM-1119 | KAT6A HAT inhibitor |
Beyond Products: Tebubio as Your Research Partner
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Broad portfolio of validated antibodies covering senescence-relevant targets
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qRT-PCR reagents for SASP and senescence gene expression analysis
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Biomarker Quantification Services to accelerate complex projects
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Local scientific support teams to help design robust, multi-parametric workflows
Discuss Your Senescence Research Strategy
Contact your nearest Tebubio office to discuss your senescence research strategy.
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References
Figures 1 & 2: Muthamil S, Kim HY, Jang HJ, Lyu JH, Shin UC, Go Y, Park SH, Lee HG, Park JH. “Biomarkers of Cellular Senescence and Aging: Current State-of-the-Art, Challenges and Future Perspectives." Adv Biol (Weinh). 2024 Sep;8(9):e2400079. doi: 10.1002/adbi.202400079. Epub 2024 Jun 27. PMID: 38935557.
Turn Cytoskeletal Insights into Reliable Results
Whether you’re optimizing an assay, validating a pathway, or planning your next experiment, Cytoskeleton tools are designed to save time while increasing confidence in your data.
Our scientific team can help you:
- Select the right assay or reagent
- Optimize workflows for your model system
- Confirm product compatibility before ordering