White DNA Helix

Transform Your Long-Read Sequencing Into a Multiomic Powerhouse

 

CUTANA™ Hia5 is the essential enzyme for Fiber-seq [1], a cutting-edge multiomic long-read sequencing (LRS) assay that simultaneously profiles chromatin accessibility, DNA methylation, and genetic variation at single-molecule resolution.

By layering chromatin accessibility on top of standard LRS workflows, Fiber-seq uniquely links epigenetic information (DNA methylation) and complex genetic architecture, including structural variants and haplotype phasing, to chromatin states within the same DNA molecules (Figure 1).

 

Figure 1: Overview of the CUTANA Fiber-Seq protocol.

How Fiber-seq Works

CUTANA™ Hia5 is a DNA adenine methyltransferase (MTase) that uses S-adenosylmethionine (SAM) to catalyse methylation at the N6 position of adenine, producing N6-methyladenine (6mA).

In Fiber-seq, Hia5’s nonspecific activity methylates adenines within accessible chromatin regions in situ, effectively recording chromatin accessibility onto native DNA without disturbing endogenous methylation (5mC).

The Hia5-modified DNA is fully compatible with long-read sequencing platforms, including PacBio® HiFi Sequencing and ONT® Nanopore Sequencing, ensuring seamless integration into your existing workflows.

 

Figure 2: Fiber-seq simultaneously detects open chromatin (6mA) and DNA methylation (5mC).

Why CUTANA™ Hia5 Is a Game-Changer

  • Multiomic insights in a single assay
    Consolidate multiple assays into one: Fiber-seq simultaneously profiles chromatin accessibility, DNA methylation, and genetic variants, reducing cost, time, and sample requirements.
  • Highest-activity 6mA-MTase
    Hia5 enables faster labelling and higher-resolution mapping of accessible DNA, with proven applications in protein footprinting, including transcription factor binding and nucleosome positioning [2–4] (refer to Figure 2).
  • Access the hardest-to-sequence regions
    Profile chromatin accessibility in repetitive and structurally complex regions such as transposable elements, centromeres, telomeres, segmental duplications, and chromosomal rearrangements.
  • Easy workflow integration
    Perform Fiber-seq in less than 2 hours, directly upstream of standard long-read sequencing library preparation (illustrated in Figure 1).
  • Resolve chromatin state heterogeneity
    Our PCR-free approach leverages long-read sequencing to profile individual DNA molecules, uncovering heterogeneity in cell populations often missed by short-read methods.

 

 

CUTANA™ Hia5: Product Specifications

Feature Details
Type Adenine Methyltransferase
Molecular Weight 33.8 kDa
Host E. coli
Epitope Tag 6xHis
Formulation 50 mM Tris pH 8.0, 1 mM DTT, 250 mM NaCl, 10% glycerol

 

FAQ

Find answers to the questions most frequently asked by our users, curated by our experts.

  • What is TriLink’s poly(A) tail modification?

    TriLink’s proprietary poly(A) modification is a small chemical moiety linked to the 3’-end of the poly(A) tail of mRNA after the in vitro transcription reaction. There are no other changes to the mRNA.

  • Do I need to change my coding sequence or DNA template to use TriLink’s poly(A) tail modification?

    No, the modification is not template-directed and occurs post-IVT. In this case, there is no need to change your DNA template, and there is no impact on the mRNA coding sequence.

  • Have you tested the tail modification with different poly(A) tail lengths and with different mRNA constructs of varying lengths?

    Yes, we have tested our proprietary poly(A) tail modification with different poly(A) tail lengths including 40A, 60A, 80A, 100A, and 120A. We have successfully added our Poly(A) tail modification to various mRNAs, such as eGFP, Fluc, mCherry, beta-gal, and Cas9 mRNA. These constructs range from 0.9 to 4.5 kb in length.

  • Do I need to change how I deliver the mRNA?

    TriLink’s poly(A) tail modification should be compatible with commonly used delivery vehicles. We have used it with common transfection reagents and LNPs, including LipofectamineTM MessengerMAXTM reagents, and ALC-0315, DSPC, Cholesterol, and DMG-PEG 2000: Pfizer/Acuitas Lipids.

  • Does the modification increase the immunogenicity or toxicity of the mRNA?

    We have not observed meaningful differences in the immunogenicity of tail-modified mRNA from non-tail-modified mRNA in our in vitro and in vivo studies.

  • Does the modification impact the integrity of my mRNA?

    No, we have data showing that the mRNA is of comparable integrity to regular oligo-dT-purified mRNA.

Tebubio: Your European Partner for mRNA Research

As TriLink’s official EU distributor, Tebubio offers more than access: we provide expert support and local convenience:

  • Scientific Expertise: Our team understands IVT and mRNA synthesis at every level. Need help designing the right protocol, selecting the optimal reagents, or troubleshooting yields? We’re here to advise.
  • Faster Delivery: Order directly through our webshop with fast delivery across Europe.
  • One-on-One Support: Talk directly to scientists, not just sales. We help researchers every day make informed product decisions.
  • Regulatory Insight: Whether you’re working on RUO, preclinical, or GMP-grade transitions, we help navigate the right path.

Agnès Marcilly, Digital Campaign Specialist

Marketing Team at Tebubio

"ModTail™ mRNA redefines performance at the molecular level — delivering longer expression, greater stability, and seamless integration into your research and therapeutic workflows."

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