Study Genetic Variants and Epigenetic Regulation in a Streamlined Workflow

CUTANA™ Fiber-seq Kit

CUTANA™ Fiber-seq is a breakthrough approach that simultaneously profiles chromatin accessibility, DNA methylation, protein footprints, and genetic variants in one long-read sequencing assay.

Unlike short-read methods, Fiber-seq resolves chromatin features in highly repetitive and structurally complex genomic regions. Fiber-seq uses a PCR-free approach to directly profile individual DNA molecules, uncovering heterogeneity in chromatin structure of cell populations. Its antibody-free workflow also enables near-nucleotide resolution footprinting of nucleosomes, transcription factors, and RNA polymerase.

With a streamlined workflow, <2-hour workflow directly upstream of standard long-read sequencing prep, Fiber-seq integrates seamlessly into existing pipelines, unlocking new insights from a single assay.

• Multiomic profiling from the same sample in one experiment
• Resolves complex, hard-to-map regions missed by short reads
• Antibody-free protein footprinting at near-nucleotide precision
• Seamless integration with existing long-read sequencing workflows
• Powered by Hia5, the ideal 6mA-MTase for protein footprinting

Figure 1: Unlike short-read techniques, Fiber-seq links genetic and epigenetic information genome-wide at single-molecule resolution, even in hard-to-map regions.

Figure 2: Simple, Scalable Workflow for Multiomic Profiling. A full detailed protocol can be downloaded here. 

Figure 3: Fiber-seq delivers multiomic insights in a single assay

Figure 4: Fiber-seq multiomic data is biologically concordant

Figure 5: Fiber-seq captures ATAC-seq like accessibility data in a long-read assay format

Figure 6: Fiber-seq captures nucleosome position + protein footprints

Unlike short-read techniques, CUTANA™ Fiber-seq links genetic and epigenetic information genome-wide at single-molecule resolution, even in hard-to-map regions.