Active Enzymes for Research & Development

Active enzymes play a central role in academic and pharmaceutical R&D, catalysing biochemical reactions that enable drug synthesis, diagnostics, and therapeutic applications.

At Tebubio, we provide a curated selection of enzymes from reputable producers, ensuring reliability, reproducibility, and top-tier activity for your experiments.

Epigenetic Enzymes

Regulate gene expression without altering the genetic code through DNA, RNA, or histone modifications. Examples include:

• Acetyltransferases

• Deacetylases (HDAC1, HDAC4, HDAC6, SIRT1…) (Figure 1)

• Methyltransferases & Demethylases

• Bromodomains (key modulators in epigenetic research)

Figure 1: Function of histone deacetylases.

Figure 2: Function of Kinases and Phosphorylases.

Kinases & Phosphorylases

Control signal transduction, cell division, and metabolism through phosphorylation. Kinases transfer phosphate groups from ATP to other molecules, while phosphorylases catalyse the reverse reaction. More than 500 known kinases control half of all cellular functions (Figure 2).

Our selection includes:

• Protein kinases (tyrosine, serine/threonine, dual kinases)

• JAK1 Kinase (Figure 3)

• PI4KIIIb (Figure 4)

• Diverse phosphatases

Figure 3: Inhibition of JAK1 kinase activity by the inhibitors ruxolitinib, JAK3-IN-6 and stauroporine.

Figure  4: The PI(4)P product of recombinant full-length human PI4KIIIb enzyme (117P32-102G) was measured in a PI 4 kinase Activity ELISA. The enzyme reactions were run for 0 and 4 hrs at RT with shaking.

PARPs & PAR Hydrolases

Key players in DNA damage response, ensuring genome integrity.
We offer PARPs and hydrolases such as PARG and ARH3 (Figure 5).

Figure 5: PARP pathway.

Phosphodiesterases (PDEs)

Catalyse hydrolysis of phosphodiester bonds in nucleotides, DNA/RNA, and lipids. Examples include PDE1a (Figure 6), PDE3B (human, rodent, dog), and many others.

Figure 6: PDE1A inhibition measured by a fluorescence polarisation assay.

Proteases

Discover a wide variety of proteases with diverse cleavage specificity for protein research, including Aminopeptidases, Carboxypeptidases, and Dipeptidyl-Peptidases (Figure 7).  

Figure 7: Diverse protease types with different cleavage sites.

Ubiquitination Enzymes

Explore the ubiquitin-proteasome system with a wide range of active enzymes related to ubiquitination: E1, E2, E3 ligases, deubiquitinases, and multi-catalytic protease complexes (Figure 8).

Figure 8: The Ubiquitin-Proteasome System: An Overview.

Functional Assays & Substrates

Need to measure the inhibitory effects of specific compounds? We offer:

• Ready-to-use functional assays for many active enzymes

• Substrates for enzyme reactions

• Full lab services to run functional assays and test inhibitory potential

For example, HDAC1 activity can be measured using the HDAC1 Fluorogenic Assay Kit (Figure 9).

 Figure 9: HDAC1 enzyme activity measured using the HDAC1 Fluorogenic Assay Kit.