Optimal knock-down can be done by a shRNA sequence that depends on the gene expression level and the cell type. Usually, 4 shRNA should be tested to find the one inducing a minimum of 70% increase of the

ORF expression in Mammalian cells is very useful for many applications: protein production with HEK293, over-expression of tagged protein for immuno-tracking or immuno-precipitation, development of a cell

In a previous post, I introduced a complete panel of ready-to-use specialized media for smooth muscle primary cells culture. Produced by Cell Applications, a GMP certified

Gene knock-out, which is gene editing leading to loss of function, just requires delivering into cells the 2 CRISPR system actors: the CAS9 endonuclease and the specific guide RNA to target

Real time visualization of cellular events by Live Cell Imaging (LCI) has led to a better understanding of the global dynamic biological process. In a constantly evolving field, tebu-bio

This month, a new leap forward in gene editing has been published in Nature under the title 'Search-and-replace genome editing without double-strand breaks or donor DNA'. DOI: 10.1038/s41586-019-1711-4.

One of the challenges in respiratory research in general, and for COVID-19 related projects in particular, is to have access to relevant in vitro cell culture models. Human lung

To overcome current cell model limitations, bit.bio have developed a new, innovative technology to reprogram human stem

With the great success of the SIR-probes for efficient labeling of the cell Cytoskeleton, DNA and Lysosome, Spirochrome have just

Since its emergence in 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has rapidly spread to become the major health issue of the past two years. But it's also a research field of interest

Many researchers are facing the challenges of mRNA delivery, through the body into the cytoplasm of cells. In this post, we invite you to discover some useful and efficient solutions to

Small molecules – overcome the barriers to clinical development

The potential of nanomedicine to provide therapeutic benefits over conventional drug formulations has been proven with a number of