Ac-VEID-AFC (Caspase 6 (Mch2) Substrate) - 25 mg

Molecular Weight: 727.27Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Val-Glu-Ile-Asp-AFCSequence (1-letter): Ac-VEID-AFCStorage: -20 °C or belowSolubility: 20 mM in DMSO or DMFAc-VEID-AFC is a fluorogenic substrate for caspase 6 (Mch-2α). Caspase 6 is a cysteine protease involved in the execution phase of apoptosis mediating neuronal shrinkage. In addition it has important roles in neurodegenerative disorders such as Huntington’s Parkinson’s and Alzheimer’s diseases. Caspase 6 cleaves the mutated huntintin protein and the amyloid precursor protein resulting in the aggregating protein fragments typical of Huntington’s and Alzheimer’s diseases respectively.Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.References1. LeBlanc A. et al. (1999) “Caspase-6 Role in Apoptosis of Human Neurons Amyloidogenesis and Alzheimer’s Disease” J. Biol. Chem. 274: 23426-23436.