Ac-Val-Asp-Val-Ala-Asp-AFC (Caspase 2 (ICH-1) Substrate) - 5 mg

CAS Number: 219137-85-6 Molecular Weight: 770.27 Salt Form: N/A Purity: >96% Sequence (3-letter): Ac-Val-Asp-Val-Ala-Asp-AFC Sequence (1-letter): Ac-VDVAD-AFC Storage: -20 °C or belowAc-Val-Asp-Val-Ala-Asp-AFC is a fluorogenic substrate for caspase 2. Caspase 2 part of the ICH-1 sub-family is a cysteine protease with a poorly defined role in apoptosis. It associates with several apoptotic proteins such as death effector filament-forming Ced-4-like apoptosis protein (DEFCAP) RIP-associated Ich-1/Ced-3-homologue protein with a death domain (RAIDD) and apoptosis repressor with caspase recruitment domain (ARC). Caspase 2 can be activated by the PIDDosome formed by the association of PIDD1 and CRADD. Recent work demonstrates that Caspase-2 resides in the mitochondria and is essential for stress-induced apoptosis. Caspase-2 cleaves at an Asp residue with a preferred sequence of Val-Asp-Val-Ala-Asp-X.Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin