Ac-Tyr-Val-Ala-Asp-pNA (Caspase 1 (ICE) Substrate) - 5 mg

CAS Number: 149231-66-3Molecular Weight: 628.25Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Tyr-Val-Ala-Asp-pNASequence (1-letter): Ac-YVAD-pNAStorage: -20 °C or belowSolubility: DMSO to 10 mM; soluble in aqueous base (e.g. 0.1 N NH4OH) to 1 mg/mLCaspase 1 (ICE) Substrate (Ac-Tyr-Val-Ala-Asp-pNA) is a substrate for interleukin 1b converting enzyme (ICE) which is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein which is a potent inhibitor of apoptosis inhibits activation of these proteases in BRK cellsKm = 23 μM (Casp-1) 29 000 μm (Casp-3) 874 μM (Casp-4)Activity is quantified by the release of p-nitroaniline which is measured by absorbance at 405 nm.References1. Alcamí A Smith