Ac-Trp-Glu-His-Asp-AMC (Caspase 1 (ICE) Substrate) - 25 mg

CAS Number: 189275-74-9 Molecular Weight: 784.79 Salt Form: TFA Purity: >96% Sequence (3-letter): Ac-Trp-Glu-His-Asp-AMC Sequence (1-letter): Ac-WEHD-AMC Storage: -20 °C or belowAc-Trp-Glu-His-Asp-AMC is a fluorogenic substrate for Caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein which is a potent inhibitor of apoptosis inhibits activation of these proteases in BRK cells. Ac-WEHD-AMC is cleaved more efficiently than Ac-YVAD-AMC.Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.