Ac-Trp-Glu-His-Asp-AFC (Caspase 1 Substrate) - 5 mg

Molecular Weight: 838.76Salt Form: N/APurity: >96%Sequence (3-letter): Ac-Trp-Glu-His-Asp-AFCSequence (1-letter): Ac-WEHD-AFCStorage: -20 °C or belowAc-WEHD-AFC is a fluorogenic substrate for caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that preferentially cleaves substrates built on VAD (val-ala-asp) or WEAD (trp-glu-ala-asp) sequences. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein which is a potent inhibitor of apoptosis inhibits activation of these proteases in BRK cells.Activity is quantified by release of free fluorescent 7-amino-4-trifluoromethylcoumarin (AFC) which excites at 395-400 nm and emits at 495-505 nm.