Ac-Trp-Glu-Ala-Asp-AMC (Caspase 1 (ICE) Substrate) - 5 mg

Molecular Weight: 718.26 Salt Form: N/A Purity: >96% Sequence (3-letter): Ac-Trp-Glu-Ala-Asp-AMC Sequence (1-letter): Ac-WEAD-AMC Storage: -20 °C or belowAc-Trp-Glu-Ala-Asp-AMC is a fluorogenic substrate for Caspase 1. Caspase 1/Interleukin 1b converting enzyme (ICE) is a cysteine protease that cleaves specifically at aspartic acid. ICE converts inactive IL-1 beta precursor to a pro-inflammatory cytokine that mediates a wide range of immune and inflammatory response. ICE-related cysteine proteases are required for E1A-induced p53-dependent apoptosis in baby rat kidney (BRK) cells. Adenovirus E1B 19K protein which is a potent inhibitor of apoptosis inhibits activation of these proteases in BRK cells.Activity is quantified by release of free fluorescent 7-amino-4-methylcoumarin (AMC) which excites at 360-380 nm and emits at 440-460 nm.