5' PtdIns(3 4 5)P3 Phosphatase Activity Fluorescence Polariz - EA KIT

The 5' PtdIns(3 4 5)P3 Phosphatase Activity Fluorescence Polarization Assay determines activity through quantification of the end product PI(3 4)P2.Sample Type: in vitro determination of 5' phosphatase activity in cancer tissues and cell lines and compound screening for drug discoverySample Volume: 2.5-5.0 µL enzyme / inhibitorAssay Range: 0.0625 µM to 2.0 µMProduct Background: This 384 well fluorescence polarization-based competitive assay measures the phosphatase activity of SHIP2 which hydrolyzes the 5-phosphate from PtdInd(3 4 5)P3. After the phosphatase reactions are complete reaction products are mixed with a PI(3 4)P2 detector protein and the fluorescent PI(3 4)P2 probe. Polarization (mP) values decrease as probe binding to the PI(3 4)P2 detector is displaced by PI(3 4)P2 produced by enzymatic activity and the amount of unbound fluorescent probe in the mixture increases.Product Keywords: SHIP2 SHIP1 PI(3 4)P2Note to Purchaser: Echelon Biosciences products are sold for