Lambda High Methylated DNA (5 µg per vial at 100 ng/µL) - 5 µg per vial

The Lambda High Methylated DNA Control is provided as 5 µg per vial at a concentration of 100 ng/µL. This control consists of lambda phage DNA that has been extensively methylated in vitro, primarily at CpG sites, to achieve near-complete methylation across the DNA sequence. This control is highly suited for epigenetic research applications, serving as a reliable standard for validating and optimizing DNA methylation assays. The applications include: Assay Calibration: Ideal for use in creating defined mixtures of methylated and non-methylated DNA, allowing for precise calibration of methylation assays and supporting accurate quantitative analysis. Spike-in Control: Suitable as a “spike-in” control: it allows researchers to assess bisulfite conversion efficiency within the same reaction as the sample, ensuring complete conversion of unmethylated cytosines in real time. Note: Bisulfite or enzymatic modification is required before use. The provided 5 µg (5000 ng) is generally sufficient