FIB growth medium, basal - 500 ml

Fibroblast Growth Medium is a sterile liquid culture medium designed for cultivating human fibroblasts (HFIB). Provided as a basal medium, it is ideal for culturing Provitro HFIB upon the addition of supplement mix components. Optimized for initial seeding of 4,000 cells/cm², it supports growth up to confluence (around 90%) without requiring feeder layers, matrix substrates, or additional substances. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. Fibroblast growth medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time. Storge: Store the supplemented Fibroblast growth medium in a dark envi