CheKine™ Micro Nitrate Reductase (NR) Activity Assay Kit - 96 T

Nitrate reductase (NR, EC1.7.1.3) exists widely in plants. It is not only the key enzyme for the conversion of nitrate nitrogen to ammonia nitrogen, but also an inducing enzyme, which has an effect on the yield and quality of crops. CheKine™ Micro Nitrate Reductase (NR) Activity Assay Kit allows for the detection of nitrate reductase activity in plant tissues, bacterial, or cellular samples. The principle behind this assay involves NR catalyzing the reduction of nitrate to nitrite, following the reaction:NO3ˉ+NADH+H＋ →NO2ˉ+NAD＋ +H2O. The resulting nitrite, under acidic conditions, then reacts quantitatively with p-amino benzenesulfonic acid and α-naphthylamine to produce a red azo compound. This red azo compound exhibits a maximum absorption peak at 540 nm, which can be measured by spectrophotometry, thereby enabling the quantification of nitrate reductase activity in the sample.