TR-FRET Based In Vitro E3 Ligase Customizable Kit - 1 kit

The TR-FRET In vitro E3 activity kit has been developed to be simple, fast, and easy to use while being robust and sensitive. As E3 ligases autoubiquitinate they build long polyubiquitin chains; our unique assay will detect these chains as they are being built. This assay is homogenous and compatible for High-throughput Screening (HTS). Choose 1 E2 option among: UBE2D3 (UbcH5c), UBE2L3 (UbcH7), UBE2K (UbcH1), Ubc13, Mms2 (yeast), UBE2D2 (UbcH5b, E2-17K2), UBE2R1 (CDC34), UBE2R2 (Ubc3B), Non-tagged UBE2D1, His6-SUMO tagged UBE2D1, UBE2E2 (UbcH8), UBE2E3 (UbcH9), His6-tagged UBE2H (E2-20K), His6-SUMO tagged UBE2H (E2-20K), UBE2F (NCE2), UBE2M (Ubc12), UBE2N (Ubc13), Non-tagged UBE2T (PIG50), His6-SUMO tagged UBE2T (PIG50), UBE2V2 (MMS2, UEV-2), UBE2S (E2EPF), UBE2C (Ubc10), His6-tagged UBE2W (Ubc 16), UBE2Q2, His6-tagged UBE2A, Non-tagged UBE2G2 (Ubc7), His6-SUMO tagged UBE2G2 (Ubc7), His6-tagged UBE2I (Ubc9), UBE2L6 (UbcH8), UBE2Z (Use1) And Choose 1 E3 option among: ITCH, Traf6, GP78,