pY-SUMOstar Saccharomyces Intracellular Expression Kit - 500 Reactions

In eukaryotes, endogenous SUMO proteases rapidly cleave SUMO fusions in vivo, precluding tag-enhanced expression and purification capacities. To circumvent this problem, LifeSensors has engineered the SUMOstar fusion system. SUMOstar offers the same solubility and expression enhancing benefits as the original wild-type SUMO tag; however, the tag is not cleaved by endogenous eukaryotic SUMO proteases. In addition, LifeSensors has created a cognate SUMOstar protease for specific  recognition and cleavage of the SUMOstar tag in vitro, liberating the protein-of-interest with a desired N-terminal amino acid. The pY-Saccharomyces Intracellular SUMOstar Kit allows for expression of SUMOstar fusion proteins intracellularly.-\n

Components:

-\n-- pY-SUMOstar vector (Cat.# 2101) — 20 µg-\n-- SUMOstar Protease 1 (Cat.# 4110) — 500 Units-\n-- SUMOstar Protease control protein (Cat. #5010) — 100 µg-\n-- Anti-SUMO/SUMOstar antibody (Cat.# AB7002) — 50 µg