pP-Pichia Secretory SUMOpro3 Expression Kit - 500 Reactions

SUMOpro3 Fusion Protein Technology The use of SUMOpro3 as a fusion system can dramatically enhance overall protein production through enhanced protein translation, solubility and stability. Each SUMO-fusion system requires a specific protease for removal of the fusion partner — for SUMOpro3 use SUMO Protease 2.  SUMO proteases are highly specific and robust enzymes that recognize and bind to the tertiary structure of SUMO in order to correctly position the C-terminal cleavage site in the enzymes active site.  With the exception of proline, these proteases will cleave your fusion protein precisely between the C-terminus of SUMO and the N-terminus of the target protein.  This requirement for the tertiary structure of SUMO virtually eliminates the ragged ends and non-specific cleavage seen with other proteases.