NEDD8-CHOP2 Reporter System - 30 Reactions

The NEDD8-CHOP-Reporter DeNEDDylation Assay Kit consists of NEDD8 fused to a reporter enzyme and the reporter enzyme substrate. When fused to NEDD8, the reporter is rendered catalytically inactive. Following cleavage of the NEDD8-reporter system by isopeptidase activity, the free (and now active) reporter enzyme subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme’s substrate is a quantitative measure of isopeptidase activity.-\n

Contents

-\n-- NEDD8-CHOP2-Reporter (Reporter System): 1 x 375 μL (4 μM)-\n-- Den1 (Control Isopeptidase): 2 x 10 μL (2 μM)-\n-- Reporter Substrate 2: 1 x 30 μL (25 μM)-\n-- Control Fluorophore 2: 1 x 20 μL (1 mM)