CUTANA™ Quick Cleanup DNA Purification Kit - 48 CUT&RUN -OR- 96 CUT&Tag Reactions

The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA. In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio. In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher CU