Scientific Library
Soluble and Insoluble Collagen assays - made easy!
The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious
2 new validated anti-PGP 9.5 antibodies for neuroscience research
Protein Gene Product 9.5 (PGP 9.5) is an abundant cytoplasmic neuron and neuroendocrine-cell specific protein. Over the years, this deubiquitinating enzyme (a.k.a. Ubiquitin Carboxy-terminal Hydrolase
Bioactive neuronal lipid mediators: a focus on Synaptamide
Focus Biomolecules produces a broad range of bioactive lipid mediators as well as many small molecule inhibitors of numerous lipid metabolic pathways for use as cellular
How to easily visualize and quantify cellular senescence
Due to its involvement in many cellular processes such as normal ageing, tumor suppression, chronic diseases and many more, cellular senescence has gained increasing attention since its first description
Quantify ExtraCellular Matrix components - 5 popular kits you need to know
Cell-Based ELISA for in-cell protein phosphorylation and expression profile detection
Cell-Based ELISA tests now enable precise measurement of the phosphorylation status or expression profiles of target proteins (biomarkers) in whole cells. In this post, a review of high performing cell-based assays is made.
Interestingly, the experimental design is simple and is based on 3 steps:
- Plate
- Treat and fix your cells of interest (adherent or in suspension)
- Detect the target proteins with the optimized reagents provided in the kit (450 nm colorimetric detection)
These Colorimetric Cell-Based ELISA Kits enable the detection of various target proteins (biomarkers). Some references also enable the precise measurement of the phosphorylation status of proteins of interest. They can easily
Neurons, Astrocytes, Microglia: High Quality primary cells source
In the field of neurosciences, increasing research is being undertaken on neurodegenerative disorders like Parkinson’s and Alzheimer’s diseases, as well as on nerve injury and regeneration. One of the
Immunoassays with collagen: Tips to optimize anti-collagen antibody performance
Dr Yong Zhang, Ph.D. is a senior scientist and R&D Manager for antibody generation and assay development at Rockland Immunochemicals, Inc. who are recognised and renowned for their high quality primary
Where to find a sensitive Hyaluronic Acid (HA) quantitative assay
Hyaluronic acid (also called HA or Hyaluronan) is a glycosaminoglycan with elevated viscosity, enabling tissues (eye, skin, joint and synovial fluid...) to resist to physical and
Consistent, scalable human iPSC-derived glutamatergic neurons and skeletal myocytes
As detailed in a separate post, bit.bio have developed a novel cellular reprogramming technology, opti-ox™
Mitochondria-Lysosome Contacts in Neurogenetic Diseases
Mitochondrial Dynamics and Mitochondria-Lysosome Contacts in Neurogenetic Diseases
In an article published in frontiers in Neuroscience, with the aim to study cellular phenotypes