Page 13 - Scientific Library
Antibody pairs - the rebirth of a classic
Antibody pairs have been used for years to detect biomarkers in biological samples. They are a convenient and price-wise alternative to ready-to-use ELISAs, especially when they are used on a routine basis
H3K4me3 Histone Demethylase Fbxl10 Activity Assay
The Histone Demethylase FBXL10 (also known as NDY1, KDM2B, or JHDM1B) has been reported to demethylate histone H3K4me3. By removing active Methyl Epigenetic marks on the chromatin, this "Epigenetic Eraser"
microRNAs: Portraying a cell - miRNA profiling
Well, in the previous posts leading up to this final one in the series, we looked at microRNA detection, overexpression, inhibition, and target detection. I'd like to finish by going over miRNA profiling.
Profiling of secretome biomarkers - pancreatic cancer
Following a post on tools for discovery of secretome biomarkers in biological samples, a recent publication by Torres et al. depicts how some of these tools (i.e. antibody arrays)
Tumour microenvironment - glioblastoma
Factors released by tumour cells can have an impact on the outcome of cancer in a patient, and they can be used as diagnostic or prognostic biomarkers. A deeper study on the tumour microenviroment (TME)
Sircol for soluble and insoluble collagen assays!
Measuring soluble collagen in biological samples is a straightforward experiment which can be done with the Sircol
Activation of RhoA, Rac1 and Cdc42 - New G-LISA Trial kits
Small GTP-binding proteins such as RhoA, Rac1, and Cdc42 are involved in regulating cell signalling pathways and impact a wide range of cellular processes, functions, and morphology. They bind and hydrolyze
miRNA mediates gene expression through HDL particles
A recent study published in Nature Communications is the first to report how a mature microRNA (miR-223) actively controls the gene expression of a cell in which it was not originally transcribed. High
Writing - Erasing - Reading Epigenetics: Targeting Erasers in Drug Discovery
DNA and Histone proteins are targets for epigenetic modifications.
One of the roles of Epigenetics is to drive cellular differentiation from totipotent Stem cells to fully differentiated cell types,
New tools to fight against Pneumococcus
Pneumococcus, or scientifically speaking, Streptococcus pneumoniae, is a pathogen responsible for a number of illnesses, including pneumonia, ear infections, sinus infections, meningitis and bacteremia.
Tumour microenvironment and miRNA biomarkers
In previous posts, we have seen the role of inflammation and glycosylation