CheKine™ Micro Acetokinase (ACK) Activity Assay Kit - 96 T

Acetokinase (ACK) is primarily found in microorganisms and functions as a key enzyme in bacterial carbon metabolism and energy metabolism, playing a central role particularly in the methanogenesis pathway of archaea. ACK catalyzes the conversion of acetate and ATP into acetyl phosphate and ADP. Subsequently, pyruvate kinase catalyzes the formation of ATP and pyruvate from ADP and PEP. Lactate dehydrogenase then catalyzes the reaction that converts pyruvate and NADH into lactate and NAD+. By measuring the rate of NADH oxidation to NAD+ at a wavelength of 340 nm, one can determine the activity of ACK.