AKAP18a-targeted cADDis cAMP Assay - 10mL - 5mL

U0261G is a fusion of the complete AKAP18a protein and the cADDis cAMP biosensor. In HEK 293 cells, the AKAP18a-targeted cADDis sensor appears to be localized to the plasma membrane and intracellular membranes. This assay is genetically encoded and packaged in Big Sky BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl).