10X Tris-Glycine (0.2 M Tris, 1.5 M Glycine, pH 8.0) - - 1 L

Tris-Glycine Running Gel buffer without SDS (sodium dodecyl sulfate) for polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins.