pUC19 High Methylated DNA (5 µg per vial at 100 ng/µL) - 5 µg per vial

The pUC19 High Methylated DNA Control, provided as 5 µg per vial at a concentration of 100 ng/µL, is composed of plasmid DNA that has been extensively methylated in vitro, primarily at CpG sites, to achieve near-complete methylation across the sequence. This control is highly suited for epigenetic research, offering a dependable standard for validating and optimizing DNA methylation assays. Applications: Assay Calibration: This control can be mixed with non-methylated DNA in defined ratios, enabling precise calibration of methylation assays and supporting accurate quantitative analysis. Spike-in Control: Ideal for use as a “spike-in” control, it helps assess bisulfite conversion efficiency within the same reaction as the sample, allowing researchers to confirm complete conversion of unmethylated cytosines in real time. Note: Bisulfite or enzymatic modification is required before use. The provided 5 µg (5000 ng) is typically sufficient for at least 500 experiments using low DNA input p