K63 TUBE (FLAG) - 50 µg

FLAG® Anti-K63 TUBE is an ideal reagent for efficient isolation and enrichment of K63-polyubiquitinated proteins from cell and tissue extracts or in vitro synthesized mixtures. Based on a peptide engineered by Sims et al., it consists of multiple ubiquitin interaction motifs (UIMs) joined by a rigid, helical linker that spaces the UIMs for selective binding to extended K63-linked polyubiquitin chains. The result is a peptide the exhibits high affinity binding to K63-linked polyubiquitin together with 1000 to 10,000-fold selectivity over K48- and K11- linkages. Expression of this peptide in vitro inhibits K63-linked polyubiquitin-dependent processes and protects K63-linked polyubiquitin chains from degradation. Combining this peptide with a FLAG epitope tag generates, for the first time, a powerful affinity reagent suitable for isolation, purification and characterization of proteins modified by K63-linked polyubiquitin. Flag K63-TUBE 1 allows isolation of K63-linked polyubiquitin