Fluo-4 Calcium Assay Kit - 1000 T

Fluo-4 AM penetrates the cell membrane and enters the cell, where it is cleaved by esterases within the cell to form Fluo-4, which is then trapped within the cell. When Fluo-4 exists as a free ligand, it is almost non fluorescent, but when combined with intracellular calcium, it can produce strong fluorescence. Laser confocal microscopy can be used to detect changes in intracellular calcium concentration.