EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1, - 50 µg

EpiDyne® Nucleosome Remodeling Assay Substrate ST601-GATC1 consists of 217 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 217 base pair DNA sequence includes an upstream 6 bp linker, the 145 bp ST601 nucleosome positioning sequence, identified by Lowary and Widom [1], and a downstream 66 bp linker (hereby named 6-N-66 nucleosome). One copy of the ‘GATC’ DNA motif is embedded within the ST601 sequence and there is a 5’ biotin-TEG group. The terminally positioned nucleosome is a suitable target for recognition and substrate for remodeling reactions by various chromatin interacting proteins.