CUTANA™ Uncharged pAG-Tn5 for CUT&Tag - 20 µL

Products in EpiCypher’s IDEA Toolbox (Innovation and Discovery of Epigenetic Applications) offer access to reagents without known or fully defined uses, enabling researchers to explore cutting-edge applications. Due to their novelty and unexplored potential, EpiCypher will engage in limited technical support. CUTANA™ Uncharged pAG-Tn5 is a fusion of proteins A and G to a highly active E. coli transposase mutant (Tn5) and is the key enzyme for CUT&Tag [1]. This product is highly purified to remove contaminating E. coli DNA. For superior normalization, as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g., EpiCypher 19-1002) are recommended. This Tn5 is uncharged and must be loaded with user-designed mosaic adapter DNA prior to use in CUT&Tag. The uncharged protein exists as a monomer but will dimerize when charged with DNA. For a charged enzyme that is ready to use, see EpiCypher 15-1017.