CheKine™ Mirco Sucrose Phosphate Synthase (SPS)  Activity As - 96 T

Sucrose is not only an important photosynthetic product but also the primary substance transported within plants and serves as a form of carbohydrate storage. Sucrose phosphate synthase (SPS, EC 2.4.1.14) uses fructose-6-phosphate as a receptor, and the formed sucrose-6-phosphate is converted into sucrose by sucrose phosphate phosphatase. The SPS-sucrose phosphate phosphatase system is generally considered the main pathway for sucrose synthesis. CheKine™ Mirco Sucrose Phosphate Synthase (SPS) Activity Assay Kit provides a simple, convenient, and rapid method for detecting SPS activity, suitable for plant tissue samples. The principle involves SPS catalyzing the formation of sucrose-6-phosphate from fructose-6-phosphate. Sucrose-6-phosphate reacts with resorcinol to produce a color change, exhibiting a characteristic absorption peak at 480 nm, with the intensity of the color being proportional to the level of enzyme activity.