CheKine™ Mirco Glyceraldehyde-3-Phosphate Dehydrogenase (GAP - 48 T

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (EC1.2.1.12) Glyceraldehyde-3-phosphoric acid catalyzed oxidation produce 1,3-diphosphate glyceric acid, is the key enzyme of glycolytic pathway, It is closely related to gluconeogenesis pathway, maintenance of blood glucose concentration in the body and the occurrence of diabetes, and plays an important role in the disorders of sugar, lipid and protein metabolism. CheKine™ Micro Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Activity Assay Kit can detect animal and plant tissues, cells or bacteria, culture solution or other liquid samples. In this kit, 3-phosphoglycerate kinase catalyzes the formation of 1,3-diphosphoglycerate from triphosphoglycerate and ATP. GAPDH reversely catalyzes 1,3 diphosphoglyceric acid and NADH to produce glyceraldehyde 3-phosphate, inorganic phosphorus and NAD+. The decrease of NADH measured at 340 nm can reflect the level of GAPDH activity.